Single cell optical imaging and spectroscopy.
Single cell optical imaging and spectroscopy.
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DOI：
10.1021/cr300336e
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发表时间：
2013-04-10
期刊：
影响因子：
62.1
通讯作者：
Fang, Ning
中科院分区：
文献类型：
--
作者：
Stender, Anthony S.;Marchuk, Kyle;Liu, Chang;Sander, Suzanne;Meyer, Matthew W.;Smith, Emily A.;Neupane, Bhanu;Wang, Gufeng;Li, Junjie;Cheng, Ji-Xin;Huang, Bo;Fang, Ning
罗伯特·胡克 (Robert Hooke) 在其 1665 年的论文《显微记录》(Micrographia) 中描述了他使用显微镜进行的许多观察，包括软木塞样本中他称之为“细胞”的隔室状结构。(1) 自胡克时代以来的三个半世纪中，显微镜和我们对细胞的理解都得到了极大的改进，目前的前景表明，显微镜和细胞之间的共生关系将继续蓬勃发展，发展到今天的水平。 可预见的未来。细胞是生物学家感兴趣的基本但复杂的“单位”，就像原子对于化学家一样。最终，当谈到解释细胞复杂的内部运作时，科学家们希望“眼见为实”，但其中存在一个复杂性。在生物系统中，看见并不总是可能的。尺寸、速度、灵敏度和其他问题困扰着想要窥探细胞内部的显微镜专家。输入各种分子和纳米颗粒探针，这些探针能够标记和精确定位在显微镜下看不见的生物成分的位置。激光、相机和成像处理技术的进步也在新兴的单细胞成像领域发挥了至关重要的作用，因为它们让人们看到了通常无法被人眼看到的快速过程。这篇综述的目的是强调过去几年单细胞光学成像领域所取得的关键进展。我们无意对正在进行的实验类型或细胞研究领域进行全面回顾。最近发表了具有明显生物学风味的评论，这些另类评论侧重于细胞的具体细节以及细胞内发生的过程。(2-7) 同样，最近也出现了讨论全谱纳米粒子探针及其特性的杰出评论论文。(6-12) 这篇评论旨在概述专门用于完成单细胞成像的工具。因此，我们在本综述的前几节中重点关注成像平台，重点关注对单细胞成像实验很重要的设计细节。接下来，我们强调特定的成像实验，这些实验强调了显微镜、纳米探针和活细胞之间可能的发现类型。特别关注与生物学兴趣的机械功能和差异化结构相关的单个探针的新兴定向和旋转跟踪。最后，我们提供了单细胞操作技术的简短但相当完整的总结。
In his 1665 treatise, Micrographia, Robert Hooke described the many observations he had made using a microscope, including compartment-like structures in cork samples that he termed “cells”.(1) In the three and a half centuries since Hooke’s day, both the microscope and our understanding of the cell have been vastly improved upon, and the current outlook suggests that the symbiotic relationship between the microscope and the cell will continue to flourish into the foreseeable future. The cell is a basic yet complicated “unit” of interest to biology, just as the atom is to chemists. Ultimately, scientists want to “see to believe” when it comes to an explanation of the complex inner workings of cells, but therein lies a complication. Seeing is not always a possibility in biological systems. Size, speed, sensitivity, and additional concerns plague the microscopist who wants to peek inside of a cell. Enter a variety of molecular and nanoparticle probes that are capable of tagging and pinpointing the location of biological components that would otherwise be invisible under the microscope. Advances in laser, camera, and imaging processing technologies have also played a crucial role in the burgeoning field of single cell imaging, because they have brought into view the fast processes that would normally escape the human eye.The purpose of this Review is to highlight the key advances that have occurred in the past several years in the field of single cell optical imaging. It is not our intent to provide a comprehensive review of the types of experiments or the areas of cell research that are ongoing. Reviews with a distinctly biological flavor have been published recently, and these alternative reviews focus on specific details of the cell and the processes that occur within.(2-7) Likewise, exceptional review papers that have discussed the full spectrum of nanoparticle probes and their properties have appeared recently.(6-12) This Review is designed to give an overview of the tools that are being specifically used to accomplish single cell imaging. As such, much of our emphasis in the first several sections of this Review is on imaging platforms, with a focus on design details that are important to single cell imaging experiments. Next, we emphasize specific imaging experiments that highlight the types of findings that are possible at the nexus of microscopy, nanoprobes, and live cells. Particular attention is paid to the emerging orientation and rotational tracking of single probes linked to mechanistic functions and differentiated structures of biological interest. Finally, we provide a brief, yet rather complete, summary of single cell manipulation techniques.
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影响因子：
3.7
作者：
Angres, Brigitte;Steuer, Heiko;Schneckenburger, Herbert
通讯作者：
Schneckenburger, Herbert
影响因子：
15
作者：
Algar, W. Russ;Wegner, David;Medintz, Igor L.
通讯作者：
Medintz, Igor L.
影响因子：
3.6
作者：
ASHKIN, A;DZIEDZIC, JM;CHU, S
通讯作者：
CHU, S
影响因子：
48
作者：
Ai, Hui-wang;Hazelwood, Kristin L.;Campbell, Robert E.
通讯作者：
Campbell, Robert E.
DOI：
10.1083/jcb.200908010
发表时间：
2010-02-08
期刊：
The Journal of cell biology
影响因子：
--
作者：
Anantharam A;Onoa B;Edwards RH;Holz RW;Axelrod D
通讯作者：
Axelrod D