Effect of laminin and cytoskeletal agents on neurite formation by NG108‐15 cells
Effect of laminin and cytoskeletal agents on neurite formation by NG108‐15 cells
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层粘连蛋白和细胞骨架剂对 NG108-15 细胞神经突形成的影响
DOI：
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发表时间：
1988
影响因子：
4.2
通讯作者：
L. Luckenbill
中科院分区：
文献类型：
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作者：
H. Kleinman;L. Luckenbill
层粘连蛋白促进神经母细胞瘤与胶质瘤杂交细胞系NG108-15的附着和过程形成。当细胞附着在层粘连蛋白上时，它们变平并保持分散，而不是成群地聚集在一起。在接触层粘连蛋白后1小时内观察到过程形成，并具有剂量依赖性。环己亚胺，一种蛋白质合成抑制剂，不阻断层粘连蛋白介导的附着和神经突的形成。与存在层粘连蛋白的细胞相比，添加解聚细胞骨架的药物导致生长在塑料上的细胞的不同行为。用长春碱或细胞松弛素处理过的塑料上的细胞既不变平也不生长。细胞加上层粘连蛋白和vinblastine收缩过程，但保持平坦，这表明层粘连蛋白诱导的过程可以通过破坏微管而不稳定。用层粘连蛋白和细胞松弛素依次处理的细胞产生薄且高度分支的突起。在层粘连蛋白底物上有高浓度细胞松弛素的细胞，即使它们的体细胞没有变平，也会形成异常过程。由于层粘连蛋白抵消了细胞松弛素对过程生长的影响，但没有改变细胞松弛素对细胞体变平的影响，因此微丝介导的细胞变平和过程形成可能涉及不同的机制。
Laminin promotes attachment and process formation in the neuroblastoma x glioma hybrid cell line NG108–15. As cells attached to laminin, they flattened and remained dispersed rather than associated in clumps. Process formation was observed within 1 hr after exposure to laminin and was dose dependent. Cycloheximide, an inhibitor of protein synthesis, did not block laminin‐mediated attachment and neurite formation. Addition of drugs that depolymerize the cytoskeleton led to different behaviors for cells grown on plastic compared with those in the presence of laminin. Cells on plastic treated with either vinblastine or cytochalasin neither flattened nor grew processes. Cells plus laminin and vinblastine retracted processes, but remained flat, suggesting that laminin‐induced processes can be destabilized by disrupting microtubules. Cells sequentially treated with laminin and cytochalasin produced processes that were thin and highly branched. Cells in high concentrations of cytochalasin on a laminin substrate formed aberrant processes even when their soma did not flatten. Since laminin counteracted the effect of cytochalasin on process outgrowth but did not alter the effect of cytochalasin on flattening of the cell body, different mechanisms mediated by microfilaments may be involved in cell flattening and in process formation.
影响因子：
2.7
作者：
ROGERS, SL;LETOURNEAU, PC;FURCHT, LT
通讯作者：
FURCHT, LT
体外睫状神经节神经元的神经突生长参数：层粘连蛋白、神经鞘瘤多聚鸟氨酸结合神经突促进因子和睫状神经营养因子的影响。
影响因子：
2.9
作者：
Davis,GE;Manthorpe,M;Varon,S
通讯作者：
Varon,S