Dissection of β-barrel outer membrane protein assembly pathways through characterizing BamA POTRA 1 mutants of Escherichia coli.
Dissection of β-barrel outer membrane protein assembly pathways through characterizing BamA POTRA 1 mutants of Escherichia coli.
复制标题
DOI：
10.1111/j.1365-2958.2010.07280.x
复制
发表时间：
2010-09
影响因子：
3.6
通讯作者：
Misra R
中科院分区：
文献类型：
--
作者：
Bennion D;Charlson ES;Coon E;Misra R
大肠杆菌BamA是介导β桶外膜蛋白（OMP）组装的异聚寡聚机制的重要组成部分。BamA的C端和n端分别折叠成跨膜β桶和5个可溶POTRA结构域。BamA POTRA 1错义和缺失突变体的详细特征揭示了两个相互竞争的OMP组装途径，其中一个是原型三聚体β-桶OMP， OmpF和LamB，并且依赖于POTRA 1。有趣的是，我们的数据表明，BamA也需要它的POTRA 1结构域才能正确组装。第二种途径独立于POTRA 1，以TolC为例。位点特异性交联分析显示，BamA的POTRA 1结构域与SurA相互作用，SurA是OmpF和LamB组装所需的周质伴侣，但不与TolC和BamA相互作用。数据表明，除了TolC外，SurA和BamA POTRA 1结构域协同作用，以协助大多数β-桶状omp的折叠和组装，TolC折叠成独特的可溶α-螺旋桶状和om锚定β-桶状。这两种组装途径最终在POTRA 1之后的某个步骤合并，但可能在膜插入之前，这被认为是由Bam A的跨膜β桶结构域催化的。
BamA of Escherichia coli is an essential component of the hetero-oligomeric machinery that mediates β-barrel outer membrane protein (OMP) assembly. The C- and N-termini of BamA fold into trans-membrane β-barrel and five soluble POTRA domains, respectively. Detailed characterization of BamA POTRA 1 missense and deletion mutants revealed two competing OMP assembly pathways, one of which is followed by the archetypal trimeric β-barrel OMPs, OmpF and LamB, and is dependent on POTRA 1. Interestingly, our data suggest that BamA also requires its POTRA 1 domain for proper assembly. The second pathway is independent of POTRA 1 and is exemplified by TolC. Site-specific cross-linking analysis revealed that the POTRA 1 domain of BamA interacts with SurA, a periplasmic chaperone required for the assembly of OmpF and LamB, but not that of TolC and BamA. The data suggest that SurA and BamA POTRA 1 domain function in concert to assist folding and assembly of most β-barrel OMPs except for TolC, which folds into a unique soluble α-helical barrel and an OM-anchored β-barrel. The two assembly pathways finally merge at some step beyond POTRA 1 but presumably before membrane insertion, which is thought to be catalyzed by the trans-membrane β-barrel domain of Bam A.
登录
查看更多内容
影响因子：
4.8
作者：
Doerrler, WT;Raetz, CRH
通讯作者：
Raetz, CRH
影响因子：
5.6
作者：
Johansen, Jesper;Rasmussen, Anders Aamann;Valentin-Hansen, Poul
通讯作者：
Valentin-Hansen, Poul
影响因子：
56.9
作者：
Kim, Seokhee;Malinverni, Juliana C.;Kahne, Daniel
通讯作者：
Kahne, Daniel
影响因子：
2.9
作者：
Berven, FS;Karlsen, OA;Jensen, HB
通讯作者：
Jensen, HB
DOI：
10.1046/j.1432-1327.2000.01296.x
发表时间：
2000-05-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Molloy, MP;Herbert, BR;Gooley, AA
通讯作者：
Gooley, AA