Adeno-associated virus 2-mediated transduction and erythroid lineage-specific expression in human hematopoietic progenitor cells.
Adeno-associated virus 2-mediated transduction and erythroid lineage-specific expression in human hematopoietic progenitor cells.
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腺相关病毒 2 介导的人造血祖细胞中的转导和红系谱系特异性表达。
DOI：
10.1007/978-3-642-80207-2_7
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发表时间：
1996
影响因子：
--
通讯作者：
Yoder,MC
中科院分区：
文献类型：
--
作者：
Srivastava,A;Wang,XS;Ponnazhagan,S;Zhou,SZ;Yoder,MC
细小病毒是最小的含dna病毒之一，可感染多种脊椎动物（Sieglet al. 1985）。人类起源的两种细小病毒，非致病性腺相关病毒2 （AAV）和细小病毒B19（一种常见的人类病原体）已被广泛研究（BernsandBohenzky1987;Brownet al. 1994）。AAV需要与辅助病毒（如腺病毒或疱疹病毒）共同感染才能实现最佳复制（Berns1990），但在没有辅助病毒的情况下，AAV基因组以特定位点的方式建立潜伏感染（KotinandBerns1989;Kotinet al. 1990,1991,1992;Samulskiet al. 1991）。相比之下，B19是一种自主复制的病毒，对人类红系祖细胞具有显著的嗜性（Ozawaet al. 1986, 1987;Yaegashiet al. 1989;Srivastavaand Lu 1988;Takahashiet al. 1990）。我们描述了重组AAV-B19杂交基因组的构建，其中我们结合了这两种细小病毒的显著特征，并推测这种杂交载体可能被证明可用于高效转导原代人造血祖细胞（Srivastavaet al. 1989）。事实上，越来越清楚的是，基于aav的载体系统可能被证明是一种有用的替代更常用的逆转录病毒和腺病毒载体，用于人类基因治疗（Muzyczka1992;Carter1993;Srivastava1994）。尽管取得了这些进展，但与AAV相关的一些基本问题仍未得到解答。例如，病毒组装的分子细节和病毒进入宿主细胞的机制还没有得到严格的分析。此外，获得aav转导基因的组织特异性表达的可行性尚未得到充分解决。在这里，我们提供的实验证据表明，载体组装需要一个精确的信号机制，并且AAV感染人类细胞是受体介导的。我们还记录了在AAV-B19杂交载体介导的人原代造血祖细胞转导后红系的限制性表达。阐明AAV生物学这些方面的分子细节将对AAV作为人类基因治疗载体的潜在应用具有重要意义。
Parvoviruses are among the smallest of the DNA-containing viruses that infect a wide variety of vertebrates (Sieglet al. 1985). Two parvoviruses of human origin, the nonpathogenic adeno-associated virus 2 (AAV) and the parvovirus B19, a common human pathogen, have been studied extensively (BernsandBohenzky1987;Brownet al. 1994). AAV requires coinfection with a helper virus, such as adenovirus or herpesvirus, for its optimal replication (Berns1990), but in the absence of a helper virus, the AAV genome establishes a latent infection in a site-specific manner (KotinandBerns1989;Kotinet al. 1990, 1991, 1992;Samulskiet al. 1991). B19, by contrast, is an autonomously replicating virus with a remarkable tropism for human erythroid progenitor cells (Ozawaet al. 1986, 1987;Yaegashiet al. 1989;Srivastavaand Lu 1988;Takahashiet al. 1990). We have described the construction of a recombinant AAV-B19 hybrid genome, in which we combined the remarkable features of these two parvoviruses, and speculated that such a hybrid vector may prove useful for high efficiency transduction of primary human hematopoietic progenitor cells (Srivastavaet al. 1989). Indeed, it has become increasingly clear that the AAV-based vector system may prove to be a useful alternative to the more commonly used retroviral and adenoviral vectors for its potential use in human gene therapy (Muzyczka1992;Carter1993;Srivastava1994). Despite these advances, a number of fundamental questions related to AAV remain unanswered. For example, the molecular details of viral assembly and the mechanism of viral entry into the host cell have not been rigorously analyzed. Furthermore, the feasibility of obtaining tissue-specific expression of an AAV-transduced gene has not been adequately addressed. Here, we provide experimental evidence to suggest that the vector assembly requires a precise signaling mechanism and that AAV infection of human cells is receptor-mediated. We also document erythroid lineage restricted expression following AAV-B19 hybrid vector-mediated transduction of primary human hematopoietic progenitor cells. Elucidation of the molecular details of these aspects of AAV biology will have important implications in the potential use of AAV as a vector in human gene therapy.
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P 血型系统：免疫化学和遗传学的最新进展。
DOI：
--
发表时间：
1981
期刊：
Seminars in hematology (Print)
影响因子：
--
作者：
Marcus Dm;Kundu Sk;A. Suzuki
通讯作者：
A. Suzuki
DOI：
10.1073/pnas.87.6.2211
发表时间：
1990-03-01
影响因子：
11.1
作者：
KOTIN, RM;SINISCALCO, M;BERNS, KI
通讯作者：
BERNS, KI
人细小病毒 B19 在胎肝红系细胞原代培养物中的繁殖
影响因子：
5.4
作者：
N. Yaegashi;H. Shiraishi;T. Takeshita;M. Nakamura;A. Yajima;K. Sugamura
通讯作者：
K. Sugamura
腺相关病毒 2 介导的小鼠造血祖细胞基因转移。
影响因子：
2.6
作者：
Zhou,SZ;Broxmeyer,HE;Cooper,S;Harrington,MA;Srivastava,A
通讯作者：
Srivastava,A
针对 P 血型抗原（球苷）特异的鼠单克隆 IgM 抗体
影响因子：
6.5
作者：
A. E. G. Kr. vondem Borne;M. J. Bos;N. Joustra;J. Tromp;R. van Wijngaarden;P. Tetteroo
通讯作者：
P. Tetteroo