B7-H1 expression is associated with poor prognosis in colorectal carcinoma and regulates the proliferation and invasion of HCT116 colorectal cancer cells.
B7-H1 expression is associated with poor prognosis in colorectal carcinoma and regulates the proliferation and invasion of HCT116 colorectal cancer cells.
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B7-H1表达与结直肠癌不良预后相关并调节HCT116结直肠癌细胞的增殖和侵袭
DOI：
10.1371/journal.pone.0076012
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Wen WH
中科院分区：
文献类型：
--
作者：
Shi SJ;Wang LJ;Wang GD;Guo ZY;Wei M;Meng YL;Yang AG;Wen WH
关于结肠直肠癌细胞中B7-H1表达的研究处于早期阶段。目前还不清楚B7-H1表达是否可能在结直肠癌中具有诊断或预后价值。此外，B7-H1如何与结直肠癌的临床特征相关尚不清楚。为了探讨B7-H1与大肠癌的关系，我们分析了B7-H1在临床标本和HCT 116细胞中的表达及其作用。采用免疫组化方法检测143例符合条件的患者石蜡包埋标本中CD 274的表达。我们还研究了B7-H1本身是否可能与结肠癌HCT 116细胞的增殖、凋亡、迁移和侵袭有关。我们的研究结果表明，B7-H1在大肠癌中高表达，并与细胞分化状态和TNM（肿瘤淋巴结转移）阶段显着相关。B7-H1表达阳性者生存期有缩短的趋势。通过多因素分析，我们证明B7-H1阳性表达是结直肠癌预后的独立预测因子。我们的研究结果表明，B7-H1沉默siRNA抑制细胞增殖，迁移和侵袭。此外，B7-H1抑制也增加了细胞凋亡。B7-H1阳性表达是预测大肠癌预后的独立指标。此外，敲低B7-H1可以抑制细胞增殖、迁移和侵袭。
The investigation concerning the B7-H1 expression in colorectal cancer cells is at an early stage. It is unclear whether B7-H1 expression may have diagnostic or prognostic value in colorectal carcinoma. Additionally, how B7-H1 is associated with the clinical features of colorectal carcinoma is not known. In order to investigate the relationship between B7-H1 and colorectal cancer, we analyzed B7-H1 expression and its effect in clinical specimens and HCT116 cells. Paraffin-embedded specimens from 143 eligible patients were used to investigate the expression of CD274 by immunohistochemistry. We also examined whether B7-H1 itself may be related to cell proliferation, apoptosis, migration and invasion in colon cancer HCT116 cells. Our results show that B7-H1 was highly expressed in colorectal carcinoma and was significantly associated with cell differentiation status and TNM (Tumor Node Metastasis) stage. Patients with positive B7-H1 expression showed a trend of shorter survival time. Using multivariate analysis, we demonstrate that positive B7-H1 expression is an independent predictor of colorectal carcinoma prognosis. Our results indicate that B7-H1 silencing with siRNA inhibits cell proliferation, migration and invasion. Furthermore, cell apoptosis was also increased by B7-H1 inhibition. Positive B7-H1 expression is an independent predictor for colorectal carcinoma prognosis. Moreover, knockdown of B7-H1 can inhibit cell proliferation, migration and invasion.
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影响因子：
24.5
作者：
Betts G;Jones E;Junaid S;El-Shanawany T;Scurr M;Mizen P;Kumar M;Jones S;Rees B;Williams G;Gallimore A;Godkin A
通讯作者：
Godkin A
影响因子：
2.2
作者：
Rajput, Ashwani;Martin, Ivan Dominguez San;Wang, Jing
通讯作者：
Wang, Jing
影响因子：
11.5
作者：
Konishi, J;Yamazaki, K;Nishimura, M
通讯作者：
Nishimura, M
影响因子：
2.4
作者：
Eppihimer, MJ;Gunn, J;Leonard, JP
通讯作者：
Leonard, JP
DOI：
10.1158/1078-0432.ccr-11-2941
发表时间：
2012-04-01
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
Hatam LJ;Devoti JA;Rosenthal DW;Lam F;Abramson AL;Steinberg BM;Bonagura VR
通讯作者：
Bonagura VR