Epstein-Barr viral DNA in acute large granular lymphocyte (natural killer) leukemic cells.
Epstein-Barr viral DNA in acute large granular lymphocyte (natural killer) leukemic cells.
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急性大颗粒淋巴细胞（自然杀伤）白血病细胞中的 Epstein-Barr 病毒 DNA。
DOI：
10.1182/blood.v79.8.2116.bloodjournal7982116
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发表时间：
1992
期刊：
影响因子：
20.3
通讯作者：
M. Beard
中科院分区：
文献类型：
--
作者：
Dnj Hart;BW Baker;MJ Inglis;JC Nimmo;GC Starling;E. Deacon;M. Rowe;M. Beard
一名男性高加索人的血清学研究显示，急性肝炎样疾病与外周血大颗粒淋巴细胞（LGL）增加有关，提示慢性或反应性EB病毒（EBV）感染。LGL显示具有自然杀伤（NK）细胞，CD 3-CD 16-CD 56 + CD 57-表型，并介导强的非特异性主要组织相容性复合物非限制性（NK）细胞毒活性。外周血LGL计数的进行性增加与快速恶化、肝坏死和死亡相关。广泛的器官浸润与LGL提示恶性淋巴组织增生性疾病，但没有淋巴（T细胞受体或IgH）基因重排或细胞遗传学标记检测。然而，分子分析鉴定了EBV基因组DNA在LGL内以单一附加体形式存在，确立了LGL增殖的克隆性质。通过原位杂交研究证实EBV感染了白血病LGL，结果显示LGL内有EBV RNA。LGL蛋白提取物的免疫印迹确定，在EBV基因产物中，表达EBV核抗原-1（EBNA-1），但在白血病细胞中未检测到EBNA-2和潜伏膜蛋白（LMP-1）。这些结果表明，EBV可能直接参与LGL细胞转化，在一种类似的方式与EBV相关的B细胞淋巴瘤，虽然其他分子的变化可能有助于一个完全恶性白血病克隆的演变。
Serologic studies in a male Caucasian presenting with an acute hepatitis-like illness, associated with an increase in peripheral blood large granular lymphocytes (LGLs), suggested a chronic or reactived Epstein-Barr virus (EBV) infection. The LGL were shown to have a natural killer (NK) cell, CD3- CD16- CD56+ CD57- phenotype and mediated strong nonspecific major histocompatability complex-unrestricted (NK) cytotoxic activity. A progressive increase in the peripheral blood LGL count was associated with a rapid deterioration, hepatic necrosis, and death. Widespread organ infiltration with LGLs suggested a malignant lymphoproliferative condition, but no lymphoid (T-cell receptor or IgH) gene rearrangement or cytogenetic marker was detected. However, molecular analysis identified EBV genomic DNA present in a single episomal form within the LGL, establishing the clonal nature of the LGL proliferation. Confirmation that the EBV had infected the leukemic LGL was obtained by in situ hybridization studies that showed EBV RNA within the LGLs. Immunoblotting of LGL protein extracts established that, of the EBV gene products, EBV nuclear antigen-1 (EBNA-1) was expressed but EBNA-2 and the latent membrane protein (LMP-1) were not detectable in the leukemic cells. These results suggest that EBV may be involved directly in LGL cell transformation, in a manner similar to EBV-associated B-cell lymphomas, although other molecular changes probably contribute to the evolution of a fully malignant leukemic clone.
EB 病毒感染的基本病毒学方面。
影响因子：
3.6
作者：
Thorley-Lawson,DA
通讯作者：
Thorley-Lawson,DA
影响因子：
158.5
作者：
BIRON, CA;BYRON, KS;SULLIVAN, JL
通讯作者：
SULLIVAN, JL