Recombinant Goat VEGF164 Increases Hair Growth by Painting Process on the Skin of Shaved Mouse.

Recombinant Goat VEGF164 Increases Hair Growth by Painting Process on the Skin of Shaved Mouse.
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重组山羊 VEGF164 通过在剃毛小鼠皮肤上进行涂漆处理来促进毛发生长

DOI:
10.5713/ajas.2014.14046
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发表时间:
2014-09
期刊:
Asian-Australasian journal of animal sciences
影响因子:
--
通讯作者:
Wang Z
Wang Z
中科院分区:
其他
文献类型:
--
作者:
Bao W;Yin J;Liang Y;Guo Z;Wang Y;Liu D;Wang X;Wang Z

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为了检测山羊血管内皮生长因子(VEGF)介导的毛发再生,将内蒙古绒山羊(Capra hircus)的VEGF164全长cDNA克隆到pET-his原核表达载体中,并将​​重组质粒转入大肠杆菌BL21细胞。 32℃下0.5 mM异丙硫基-β-D-半乳糖苷诱导重组6×his-gVEGF164蛋白的表达。使用单克隆抗 his 和抗 VEGF 抗体通过蛋白质印迹纯化并鉴定重组山羊 VEGF164 (rgVEGF164)。将 rgVEGF164 涂抹到剃毛小鼠的背部区域,我们注意到该区域的毛发再生速度比对照组更快。因此,rgVEGF164 可以增加小鼠的毛发生长。
To detect goat vascular endothelial growth factor (VEGF)-mediated regrowth of hair, full-length VEGF164 cDNA was cloned from Inner Mongolia cashmere goat (Capra hircus) into the pET-his prokaryotic expression vector, and the recombinant plasmid was transferred into E. coli BL21 cells. The expression of recombinant 6×his-gVEGF164 protein was induced by 0.5 mM isopropyl thio-β-D-galactoside at 32°C. Recombinant goat VEGF164 (rgVEGF164) was purified and identi ed by western blot using monoclonal anti-his and anti-VEGF antibodies. The rgVEGF164 was smeared onto the dorsal area of a shaved mouse, and we noted that hair regrowth in this area was faster than in the control group. Thus, rgVEGF164 increases hair growth in mice.
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