Embryonic-only arsenic exposure in killifish (Fundulus heteroclitus) reduces growth and alters muscle IGF levels one year later.
Embryonic-only arsenic exposure in killifish (Fundulus heteroclitus) reduces growth and alters muscle IGF levels one year later.
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鳉鱼（Fundulushetroclitus）仅在胚胎阶段接触砷，一年后会降低生长并改变肌肉 IGF 水平。
DOI：
10.1016/j.aquatox.2017.02.020
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Bain,LisaJ
中科院分区：
文献类型：
--
作者：
Szymkowicz,DanaB;Sims,KaleighC;Castro,NoemiM;Bridges,WilliamC;Bain,LisaJ
砷是世界许多地区饮用水和农作物的污染物。流行病学研究表明，接触砷与新生儿体重下降、体重增加和适当的骨骼肌功能有关。本研究的目的是以剑鱼(Fundulus Heterocltes)为模型，研究单纯胚胎砷暴露对肌肉生长和胰岛素样生长因子(IGF)途径的长期影响。从受精到孵化，小鲤鱼胚胎暴露在0、50、200或800 ppb的AsIII中。取16、28、40、52周龄的幼鱼，在清水中饲养，采集肌肉标本。在16周、28周和40周龄时，暴露在砷中的鱼的状况因素显著减少，从12%到17%不等。然而，到52周时，条件因素没有明显变化。IGF-1R和IGF-1水平的改变被评估为生长减慢的潜在机制。虽然肝脏的IGF-1转录本没有变化，但骨骼肌细胞也可以产生自己的IGF-1和/或改变IGF-1受体的水平，以帮助促进生长。在200和800 ppb的胚胎暴露后，在清洁水中生长16周的鱼的IGF-1R转录分别是未暴露的鱼的2.8倍和2倍。在40周龄期间，200Gppb和800Gppb胚胎暴露组的IGF1-R水平保持在1.8-3.9倍的水平，而在52周龄时，800Gppb胚胎暴露组的IGF-1R水平仍然显著增加。在800ppb胚胎暴露组中，骨骼肌IGF-1转录水平在52周的水中清洁生长过程中也显著增加了1.9-5.1倍。根据这些结果，胚胎砷暴露具有长期影响，因为即使在暴露结束一年后，它也会抑制生长并增加骨骼肌中IGF-1和IGF-1R的水平。
Arsenic is a contaminant of drinking water and crops in many parts of the world. Epidemiological studies have shown that arsenic exposure is linked to decreased birth weight, weight gain, and proper skeletal muscle function. The goal of this study was to use killifish (Fundulus heteroclitus) as a model to determine the long-term effects of embryonic-only arsenic exposure on muscle growth and the insulin-like growth factor (IGF) pathway. Killifish embryos were exposed to 0, 50, 200 or 800 ppb AsIIIfrom fertilization until hatching. Juvenile fish were reared in clean water and muscle samples were collected at 16, 28, 40 and 52 weeks of age. There were significant reductions in condition factors, ranging from 12 to 17%, in the fish exposed to arsenic at 16, 28 and 40 weeks of age. However, by 52 weeks, no significant changes in condition factors were seen. Alterations in IGF-1R and IGF-1 levels were assessed as a potential mechanism by which growth was reduced. While there no changes in hepatic IGF-1 transcripts, skeletal muscle cells can also produce their own IGF-1 and/or alter IGF-1 receptor levels to help enhance growth. After a 200 and 800 ppb embryonic exposure, fish grown in clean water for 16 weeks had IGF-1R transcripts that were 2.8-fold and 2-fold greater, respectively, than unexposed fish. Through 40 weeks of age, IGF1-R remained elevated in the 200 ppb and 800 ppb embryonic exposure groups by 1.8–3.9-fold, while at 52 weeks of age, IGF-1R levels were still significantly increased in the 800 ppb exposure group. Skeletal muscle IGF-1 transcripts were also significantly increased by 1.9–5.1 fold through the 52 weeks of grow-out in clean by water in the 800 ppb embryonic exposure group. Based on these results, embryonic arsenic exposure has long-term effects in that it reduces growth and increases both IGF-1 and IGF-1R levels in skeletal muscle even 1 year after the exposure has ended.
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影响因子：
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作者：
Ambrosio, Fabrisia;Brown, Elke;Stolz, Donna;Ferrari, Ricardo;Goodpaster, Bret;Deasy, Bridget;Distefano, Giovanna;Roperti, Alexandra;Cheikhi, Amin;Garciafigueroa, Yesica;Barchowsky, Aaron
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