TGF beta inhibits expression of SP-A, SP-B, SP-C, but not SP-D in human alveolar type II cells.
TGF beta inhibits expression of SP-A, SP-B, SP-C, but not SP-D in human alveolar type II cells.
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DOI：
10.1016/j.bbrc.2018.04.003
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发表时间：
2018-05-23
影响因子：
3.1
通讯作者：
Mason RJ
中科院分区：
文献类型：
--
作者：
Correll KA;Edeen KE;Zemans RL;Redente EF;Mikels-Vigdal A;Mason RJ
转化生长因子β是一种多功能细胞因子，可调节肺泡上皮细胞、免疫细胞和成纤维细胞。转化生长因子β可抑制人胎肺表面活性蛋白A、B和C的表达，并能抑制成纤维细胞生长因子7(Fgf7，KGF)诱导的II型细胞增殖。然而，关于转化生长因子β对成人II型细胞的直接影响，我们知之甚少。我们在气/液界面条件下培养肺泡II型细胞，以维持其在有或没有转化生长因子β的情况下的分化状态。转化生长因子β显著降低SP-A、SP-B、SP-C、脂肪酸合成酶和磷脂转运蛋白ABCA3的表达。然而，转化生长因子β增加了SPD的蛋白水平，而对mRNA水平几乎没有影响，这表明它是独立于表面活性物质的其他成分进行调节的。转化生长因子β是表面活性物质的蛋白质和磷脂成分的负调节因子。转化生长因子β不能诱导高分化的人II型细胞的EMT改变。SP-D是一种重要的宿主防御分子，其调控独立于其他表面活性蛋白。综上所述，这些数据是关于转化生长因子β对高度分化的成人II型细胞影响的第一份报告。表面活性物质对肺表面活性物质系统的影响可能在纤维化肺疾病的发生发展中起重要作用。
TGF beta is a multifunctional cytokine that regulates alveolar epithelial cells as well as immune cells and fibroblasts. TGF beta inhibits surfactant protein A, B and C expression in fetal human lung and can inhibit type II cell proliferation induced by FGF7 (KGF). However, little is known about direct effects of TGF beta on adult human type II cells. We cultured alveolar type II cells under air/liquid interface conditions to maintain their state of differentiation with or without TGF beta. TGF beta markedly decreased expression of SP-A, SP-B, SP-C, fatty acid synthase, and the phospholipid transporter ABCA3. However, TGF beta increased protein levels of SPD with little change in mRNA levels, indicating that it is regulated independently from other components of surfactant. TGF beta is a negative regulator of both the protein and the phospholipid components of surfactant. TGF beta did not induce EMT changes in highly differentiated human type II cells. SP-D is an important host defense molecule and regulated independently from the other surfactant proteins. Taken together these data are the first report of the effect of TGF beta on highly differentiated adult human type II cells. The effects on the surfactant system are likely important in the development of fibrotic lung diseases.
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影响因子：
4.8
作者：
Davé, V;Childs, T;Whitsett, JA
通讯作者：
Whitsett, JA
影响因子：
5.8
作者：
Kosmider B;Messier EM;Janssen WJ;Nahreini P;Wang J;Hartshorn KL;Mason RJ
通讯作者：
Mason RJ
DOI：
10.1165/ajrcmb/5.2.155
发表时间：
1991-08-01
影响因子：
6.4
作者：
KHALIL, N;OCONNOR, RN;GREENBERG, AH
通讯作者：
GREENBERG, AH
影响因子：
4.6
作者：
Leslie, Kevin O.
通讯作者：
Leslie, Kevin O.
影响因子：
24.3
作者：
Günther, A;Schmidt, R;Seeger, W
通讯作者：
Seeger, W