A rationally designed rhodamine-based fluorescent probe for molecular imaging of peroxynitrite in live cells and tissues.
A rationally designed rhodamine-based fluorescent probe for molecular imaging of peroxynitrite in live cells and tissues.
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DOI：
10.1039/c6sc00012f
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发表时间：
2016-08-01
期刊：
影响因子：
8.4
通讯作者：
Yang D
中科院分区：
文献类型：
--
作者：
Peng T;Chen X;Gao L;Zhang T;Wang W;Shen J;Yang D
一种新的荧光报告HKYellow被合理地设计用于活细胞和组织中过氧亚硝酸根产生的稳健可视化。在此，我们介绍了一种新型罗丹明荧光探针HKYellow的合理设计、合成、表征和生物学应用，用于过亚硝酸盐的检测和分子成像，过亚硝酸盐是一种参与多种生理和病理过程的重要高活性氧化剂。基于N-苯基对罗丹明核心的高效荧光猝灭作用和过氧亚硝酸根引发的N-脱芳基反应，合理设计了HKYellow，实现了对过氧亚硝酸根在化学和生物水平上的灵敏和选择性荧光开启响应。该探针已被彻底评估为活细胞和组织中过氧亚硝酸盐的稳健成像。通过利用HKYellow，我们提供了第一个直观的证据表明，过氧亚硝酸盐在小鼠肝组织中产生的急性酒精狂欢或缺血再灌注条件下。这种探针应该是一个强大的分子成像工具，询问复杂的生物作用下的各种生物设置的过氧亚硝酸根。
A novel fluorescent reporter HKYellow was rationally designed for the robust visualization of peroxynitrite production in live cells and tissues. Herein, we present the rational design, synthesis, characterization, and biological applications of a new rhodamine-based fluorescent probe, HKYellow, for the detection and molecular imaging of peroxynitrite, an important highly reactive oxidant involved in a variety of physiological and pathological processes. HKYellow was rationally designed on the basis of the efficient fluorescence quenching effect of the N-phenyl group to the rhodamine core and a peroxynitrite-triggered N-dearylation reaction to achieve a sensitive and selective fluorescence turn-on response toward peroxynitrite in chemical and biological levels. This probe has been thoroughly evaluated for the robust imaging of peroxynitrite in live cells and tissues. By utilizing HKYellow, we provide the first visual evidence that peroxynitrite is generated in mouse liver tissues under an acute alcohol binge or ischemic–reperfusion condition. This probe should be a powerful molecular imaging tool for interrogating the complex biological roles of peroxynitrite under various biological settings.
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