Nature and mode of action of vaccinia virus products that block activation of the interferon-mediated ppp(A2'p)nA-synthetase.
Nature and mode of action of vaccinia virus products that block activation of the interferon-mediated ppp(A2'p)nA-synthetase.
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阻断干扰素介导的 ppp(A2p)nA 合成酶激活的痘苗病毒产物的性质和作用方式。
DOI：
10.1016/0042-6822(84)90269-1
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发表时间：
1984
期刊：
影响因子：
3.7
通讯作者：
M. Esteban
中科院分区：
文献类型：
--
作者：
E. Páez;M. Esteban
在这份报告中，它已经表明，在IFN处理，牛痘病毒感染的小鼠L和人HeLa S3细胞中的2-5A合成酶的抑制与特定的病毒功能有关。这种抑制作用与ATP降解和ppp（A2′p）nA去磷酸化同时发生。至少有两种病毒介导的酶活性被认为参与了这一过程，ATP酶和磷酸酶。在测定ATP水解的程度、2-5A的性质和不同寡聚体的相对丰度后确定ATP酶活性。将细胞质细胞提取物和纯化的牛痘病毒粒子与聚（I）：（C）琼脂糖结合，与[3 H]ATP、[α-32 P]ATP或[γ-32 P]ATP孵育，并通过TLC测定ATP水解的程度。通过酶和碱处理表征真实的2-5A和各种低聚物的相对丰度，并通过TLC和HPLC分析鉴定。用[γ-32 P]ATP和多核苷酸激酶标记2-5A的5′-OH末端，测定2-5A的去磷酸化程度，然后用薄层层析法测定磷酸酶活性。虽然在用来自IFN处理的未感染细胞的结合的聚（I）：（C）琼脂糖酶组分合成的寡聚体中未观察到游离的5′-OH末端，但在来自IFN处理的感染细胞的寡聚体中发现了强烈的磷酸化。这些发现表明，正是这些病毒酶活性的贡献使牛痘病毒对干扰素产生抗性。
In this report it has been shown that inhibition of the 2–5A synthetase in IFN-treated, vaccinia virus-infected mouse L and human HeLa S3cells is related to specific viral functions. This inhibition occurs concomitantly with degradation of ATP and with dephosphorylation of ppp(A2′p)nA. At least two viral-mediated enzyme activities are thought to be involved in this process, an ATPase and a phosphatase. The ATPase activity was established after determining the extent of hydrolysis of ATP, the nature of 2–5A, and the relative abundance of the different oligomers. Cytoplasmic cell extracts and purified vaccinia virions were bound to poly (I):(C) agarose, incubated with3H]ATP, [α-32P]ATP, or [γ-32P]ATP, and the extent of hydrolysis of ATP was determined by TLC. Authentic 2–5A and the relative abundance of the various oligomers were characterized by enzymatic and alkali treatments and identification by TLC and HPLC analysis. The phosphatase activity was measured by TLC after determining the degree of dephosphorylation of 2–5A from the extent of labeling at the 5′-OH termini with [γ-32P]ATP and polynucleotide kinase. While free 5′-OH termini were not observed in oligomers synthesized with bound poly (I):(C) agarose enzyme fractions from IFN-treated, uninfected cells, a strong phosphorylation was found in oligomers from IFN-treated, infected cells. These findings suggest that it is the contribution of these viral enzyme activities that renders vaccinia virus resistant to interferons.
痘苗病毒对干扰素的耐药性与病毒与干扰素系统之间的干扰现象有关。
影响因子：
3.7
作者：
Paez,E;Esteban,M
通讯作者：
Esteban,M