Characterization of AGAT, GAMT and CT1 in amphioxus: implications for the evolutionary conservation of creatine metabolism related molecules at the invertebrate-to-vertebrate transition
Characterization of AGAT, GAMT and CT1 in amphioxus: implications for the evolutionary conservation of creatine metabolism related molecules at the invertebrate-to-vertebrate transition
复制标题
文昌鱼 AGAT、GAMT 和 CT1 的表征：对无脊椎动物到脊椎动物转变过程中肌酸代谢相关分子进化保守的影响
DOI：
10.1007/s00427-008-0241-0
复制
发表时间：
2008-09
影响因子：
2.4
通讯作者：
中科院分区：
文献类型：
--
作者：
In vertebrates, glycine amidinotransferase (AGAT), guanidinoacetate methyltransferase (GAMT), and creatine transporter (CT1) are three proteins involved in creatine synthesis. To provide insight into the molecular evolution mechanism of creatine metabolism, we have cloned and identifiedBbAGAT,BbGAMT, andBbCT1homologous genes in amphioxus (Branchiostoma belcheri), whose predicted proteins show high identities with AGAT, GAMT, and CT1 proteins in vertebrates. The phylogenetic analysis indicates that amphioxus AGAT, GAMT, and CT1 are branched off at the base of the vertebrate homologous clade, respectively. Genomic structures ofBfAGAT,BfGAMT, andBfCT1show their comparability with the homologs in vertebrate and original characteristic of cephalochordate, which is consistent with animal classification. To determine the expression patterns ofBbAGAT,BbGAMT, andBbCT1, whole-mount and section in situ hybridizations are carried out in embryos and adults of amphioxus. During embryogenesis, they are all expressed mainly in mesendoderm and late somites, butBbCT1is also expressed in differentiating notochord and digestive tract, as well as in the cytoplasm of zygotes and the blastomeres at cleavage stage. In adult, the transcripts ofBbAGATandBbGAMTare detected in the neural cord, gill, nephridia, endostyle, gut, and gonads, whileBbCT1is expressed mainly in the epithelium of gut. The expression pattern of these three genes is similar to their vertebrate homologs. The result reveals that AGAT, GAMT, and CT1, the primary elements of vertebrate creatine metabolism, exist in cephalochordate amphioxus, and are highly conserved during evolution. It also suggests that similar mechanism of creatine synthesis in vertebrate may occur in amphioxus.
登录
查看更多内容
DOI：
10.1016/0006-3002(57)90241-x
发表时间：
1957
期刊：
Biochimica et Biophysica Acta
影响因子：
--
作者：
J. Roché;N. Thoai;Y. Robin
通讯作者：
J. Roché;N. Thoai;Y. Robin
DOI：
--
发表时间：
2009
期刊：
--
影响因子：
--
作者：
O. Braissant;H. Henry;A. Villard;O. Speer;T. Wallimann;C. Bachmann
通讯作者：
O. Braissant;H. Henry;A. Villard;O. Speer;T. Wallimann;C. Bachmann
影响因子：
9.8
作者：
Salomons, GS;van Dooren, SJM;Jakobs, C
通讯作者：
Jakobs, C
影响因子：
3.6
作者：
Hang Lee;Hirofumi Ogawa;Motoji Fujioka;G. Gerton
通讯作者：
Hang Lee;Hirofumi Ogawa;Motoji Fujioka;G. Gerton
影响因子：
--
作者：
P. W. Holland
通讯作者：
P. W. Holland