Protective Effect of Melatonin against Oxidative Stress-Induced Apoptosis and Enhanced Autophagy in Human Retinal Pigment Epithelium Cells.
Protective Effect of Melatonin against Oxidative Stress-Induced Apoptosis and Enhanced Autophagy in Human Retinal Pigment Epithelium Cells.
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DOI：
10.1155/2018/9015765
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发表时间：
2018
影响因子：
--
通讯作者：
Hsia SM
中科院分区：
文献类型：
--
作者：
Chang CC;Huang TY;Chen HY;Huang TC;Lin LC;Chang YJ;Hsia SM
视网膜相关性黄斑变性（AMD）影响视网膜黄斑并导致视力丧失，并且AMD是全世界老年人失明和严重视力损害的主要原因。AMD的特征在于玻璃疣在布鲁赫膜中的积累以及视网膜色素上皮（RPE）细胞和光感受器的功能障碍。AMD的发病机制尚不清楚，也没有有效的治疗方法。越来越多的证据表明，氧化应激在RPE细胞变性和AMD中起关键作用。褪黑激素是一种清除自由基的抗氧化剂，具有抗炎、抗肿瘤和抗血管生成的作用。本研究探讨了褪黑素对RPE细胞氧化损伤的抗氧化、抗凋亡和自噬作用。我们使用过氧化氢（H2 O2）刺激活性氧的产生，导致ARPE-19细胞系的细胞凋亡。我们的研究结果表明，褪黑素治疗显着抑制H2 O2诱导的RPE细胞损伤，降低凋亡率，增加线粒体膜电位，并增加自噬效应。此外，褪黑激素降低Bax/Bcl-2比值和凋亡相关蛋白细胞色素c和caspase 7的表达水平。此外，褪黑激素上调自噬相关蛋白LC 3-II和Beclin-1的表达，下调p62的表达。因此，褪黑激素对自噬和凋亡的作用可以保护人RPE细胞免受H2 O2诱导的氧化损伤。褪黑素对H2 O2诱导的人RPE细胞氧化损伤具有多重保护作用。
Age-related macular degeneration (AMD) affects the retinal macula and results in loss of vision, and AMD is the primary cause of blindness and severe visual impairment among elderly people worldwide. AMD is characterized by the accumulation of drusen in the Bruch's membrane and dysfunction of retinal pigment epithelial (RPE) cells and photoreceptors. The pathogenesis of AMD remains unclear, and no effective treatment exists. Accumulating evidence indicates that oxidative stress plays a critical role in RPE cell degeneration and AMD. Melatonin is an antioxidant that scavenges free radicals, and it has anti-inflammatory, antitumor, and antiangiogenic effects. This study investigated the antioxidative, antiapoptotic, and autophagic effects of melatonin on oxidative damage to RPE cells. We used hydrogen peroxide (H2O2) to stimulate reactive oxygen species production to cause cell apoptosis in ARPE-19 cell lines. Our findings revealed that treatment with melatonin significantly inhibited H2O2-induced RPE cell damage, decreased the apoptotic rate, increased the mitochondrial membrane potential, and increased the autophagy effect. Furthermore, melatonin reduced the Bax/Bcl-2 ratio and the expression levels of the apoptosis-associated proteins cytochrome c and caspase 7. Additionally, melatonin upregulated the expression of the autophagy-related proteins LC3-II and Beclin-1 and downregulated the expression of p62. Thus, melatonin's effects on autophagy and apoptosis can protect against H2O2-induced oxidative damage in human RPE cells. Melatonin may have multiple protective effects on human RPE cells against H2O2-induced oxidative damage.
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DOI：
10.1042/bj20111451
发表时间：
2012-01-15
期刊：
The Biochemical journal
影响因子：
--
作者：
Lee J;Giordano S;Zhang J
通讯作者：
Zhang J
影响因子：
64.5
作者：
Kim JY;Zhao H;Martinez J;Doggett TA;Kolesnikov AV;Tang PH;Ablonczy Z;Chan CC;Zhou Z;Green DR;Ferguson TA
通讯作者：
Ferguson TA
DOI：
10.1016/s0731-7085(03)00055-4
发表时间：
2003-04-24
影响因子：
3.4
作者：
Di Mambro, VM;Borin, MF;Fonseca, MJV
通讯作者：
Fonseca, MJV
影响因子：
3.4
作者：
Liang, FQ;Green, L;Godley, BF
通讯作者：
Godley, BF
影响因子：
3.9
作者：
Brennan, R.;Jan, J. E.;Lyons, C. J.
通讯作者：
Lyons, C. J.