Quantitative analysis of regulatory flexibility under changing environmental conditions.
Quantitative analysis of regulatory flexibility under changing environmental conditions.
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DOI：
10.1038/msb.2010.81
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发表时间：
2010-11-02
影响因子：
9.9
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中科院分区：
文献类型：
--
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生物钟控制许多生物过程中的24小时节律，允许相对于黎明和黄昏的生物节律的适当定时。已知的时钟电路包括多个互锁的反馈回路。理论表明，多环有助于分子节奏的灵活性，以跟踪外部循环的多个阶段。清晰的黎明和黄昏跟踪节奏说明了Ipomoea nil时间的灵活性。拟南芥中的分子钟组件表现出复杂的，光周期依赖性的调节，这是通过与三个对比模型进行比较分析。一个简单的，定量的措施，黄昏灵敏度，被引入到不同的循环复杂度的时钟模型的行为进行比较。晚上表达的时钟基因表现出光周期依赖的黄昏敏感性，预测的三环模型，而一个和两个环模型跟踪黎明和黄昏，分别。淀粉降解的输出基因通过光调节实现了黄昏跟踪表达，而不是黄昏跟踪节律。模型分析预测了哪些生化过程可以被操纵以延长黄昏跟踪。我们的研究结果揭示了生物调节器的工作原理如何专门适用于植物昼夜节律钟。
The circadian clock controls 24-h rhythms in many biological processes, allowing appropriate timing of biological rhythms relative to dawn and dusk. Known clock circuits include multiple, interlocked feedback loops. Theory suggested that multiple loops contribute the flexibility for molecular rhythms to track multiple phases of the external cycle. Clear dawn- and dusk-tracking rhythms illustrate the flexibility of timing in Ipomoea nil. Molecular clock components in Arabidopsis thaliana showed complex, photoperiod-dependent regulation, which was analysed by comparison with three contrasting models. A simple, quantitative measure, Dusk Sensitivity, was introduced to compare the behaviour of clock models with varying loop complexity. Evening-expressed clock genes showed photoperiod-dependent dusk sensitivity, as predicted by the three-loop model, whereas the one- and two-loop models tracked dawn and dusk, respectively. Output genes for starch degradation achieved dusk-tracking expression through light regulation, rather than a dusk-tracking rhythm. Model analysis predicted which biochemical processes could be manipulated to extend dusk tracking. Our results reveal how an operating principle of biological regulators applies specifically to the plant circadian clock.
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