Expression of CXCR4 on T-cell subsets and Plasma IL-17 Concentrations in Patients with Aplastic Anaemia.
Expression of CXCR4 on T-cell subsets and Plasma IL-17 Concentrations in Patients with Aplastic Anaemia.
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再生障碍性贫血患者 T 细胞亚群 CXCR4 表达及血浆 IL-17 浓度
DOI：
10.1038/s41598-017-08699-z
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发表时间：
2017-08-22
影响因子：
4.6
通讯作者：
Jiang H
中科院分区：
文献类型：
--
作者：
Niu Q;Zhou Q;Liu Y;Jiang H
获得性再生障碍性贫血（AA）是由T细胞响应趋化因子（例如，CXCR4）。我们研究了循环T细胞亚群上CXCR 4的表达、血浆IL-17 A浓度及其与AA表现的相关性。我们招募了71例获得性AA患者（36例重度AA病例[SAA]和35例非重度AA病例[NSAA]）和42例健康志愿者。我们使用流式细胞术和ELISA来测量循环中的CD 4+和CD 8 + T细胞、它们的CXCR 4表达和血浆IL-17 A浓度。与健康对照组相比，SAA患者外周血CD 4 + T细胞减少，CD 8 + T细胞增多，CD 4 +/CD 8+比值显著降低，且与AA临床表现呈正相关。SAA或NSAA患者的CD 4 + CXCR 4+和CD 8 + CXCR 4 + T细胞比例较高，与血红蛋白浓度和中性粒细胞绝对计数呈负相关。SAA和NSAA患者血浆IL-17 A浓度较高，与AA临床表现和CD 4 +/CD 8+比值呈负相关。IL-17 A浓度与CD 4 + CXCR 4 + T细胞频率显示非常弱的相关性，而与CD 8 + CXCR 4 + T细胞频率无相关性。异常CXCR 4表达可能允许循环T细胞，特别是CD 8 + T细胞，在AA进展期间浸润BM。IL-17 A浓度升高可能导致AA进展超出CXCR 4-SDF-1α轴。
Acquired aplastic anaemia (AA) is caused by T-cells migrating to and attacking bone marrow (BM) in response to chemokines (e.g., CXCR4). We investigated CXCR4 expressions on circulating T-cell subsets, plasma IL-17A concentrations, and their correlations with AA manifestations. We enrolled 71 patients with acquired AA (36 severe AA cases [SAA] and 35 non-severe AA cases [NSAA]) and 42 healthy volunteers. We used flow cytometry and ELISA to measure circulating CD4+ and CD8+ T-cells, their CXCR4 expressions, and plasma IL-17A concentrations. Compared to the healthy controls, SAA patients had fewer peripheral CD4+ T-cells, more CD8+ T-cells, and a significantly decreased CD4+/CD8+ ratio which was positively correlated with AA manifestations. Patients with SAA or NSAA had higher proportions of CD4+CXCR4+ and CD8+CXCR4+ T-cells, which were negatively correlated with haemoglobin concentrations and absolute neutrophil counts. Patients with SAA or NSAA had higher plasma IL-17A concentrations, which were negatively correlated with AA manifestations and the CD4+/CD8+ ratio. IL-17A concentrations showed a very week correlation with CD4+CXCR4+ T-cells frequencies, and no correlation with CD8+CXCR4+ T-cells frequencies. Aberrant CXCR4 expression may allow circulating T-cells, especially CD8+ T-cells, to infiltrate BM during AA progression. Elevated IL-17A concentrations may contribute to AA progression outside of the CXCR4-SDF-1α axis.
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影响因子：
--
作者：
Moll, Natalia M.;Cossoy, Michael B.;Fisher, Elizabeth;Staugaitis, Susan M.;Tucky, Barbara H.;Rietsch, Anna M.;Chang, Ansi;Fox, Robert J.;Trapp, Bruce D.;Ransohoff, Richard M.
通讯作者：
Ransohoff, Richard M.
影响因子：
3.8
作者：
Roy LD;Sahraei M;Schettini JL;Gruber HE;Besmer DM;Mukherjee P
通讯作者：
Mukherjee P
影响因子：
4.1
作者：
Siloşi I;Boldeanu MV;Cojocaru M;Biciuşcă V;Pădureanu V;Bogdan M;Badea RG;Avramescu C;Petrescu IO;Petrescu F;Siloşi CA
通讯作者：
Siloşi CA
影响因子：
20.3
作者：
de Latour, Regis Peffault;Visconte, Valeria;Young, Neal S.
通讯作者：
Young, Neal S.
IL-21 参与再生障碍性贫血的病理生理学
影响因子：
3.1
作者：
Zhang, Jizhou;Wu, Qingqing;Zheng, Yizhou
通讯作者：
Zheng, Yizhou