Genetical genomics of growth in a chicken model.
Genetical genomics of growth in a chicken model.
复制标题
鸡模型中生长的基因组学。
DOI：
10.1186/s12864-018-4441-3
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发表时间：
2018-01-23
期刊：
影响因子：
4.4
通讯作者：
Wright D
中科院分区：
文献类型：
--
作者：
Johnsson M;Henriksen R;Höglund A;Fogelholm J;Jensen P;Wright D
身体质量和生长的遗传学是理解生物学中广泛的进化和发育主题的关键。体重和生长性状受许多影响较小的遗传变异的影响。这使生长和体重的基因图谱变得复杂。来自不同群体的个体之间的实验杂交使我们能够通过连锁作图来定位选定性状的自然发生的遗传变异，例如体重。通过同时测量性状和中间分子表型，如基因表达，人们可以使用整合基因组学来寻找潜在的致病基因。本研究采用连锁定位的方法，对野生红来航鸡与家养白来航蛋鸡杂交后代的生长性状(N = 471)和肝脏基因表达(N = 130)进行了定位。通过芯片检测，我们发现了16个影响生长性状的基因座和1463个影响肝脏基因表达的基因座。其中，TRAK1、OSBPL8、YEATS4、CEP55和PIP4K2B基因被确定为鸡生长基因座的有力候选者。我们还显示了高度的性别特异性基因调控，几乎每个基因表达位点都表现出性别交互作用。最后，发现了几个跨调控热点，其中一个与一个主要的生长轨迹重合。这些发现不仅有助于确定影响生长的几个强有力的候选基因，而且还表明了性别特异性和局部基因调控如何影响鸡的生长调控。本文的在线版本(10.1186/s12864-0184441-3)包含补充材料，可供授权用户使用。
The genetics underlying body mass and growth are key to understanding a wide range of topics in biology, both evolutionary and developmental. Body mass and growth traits are affected by many genetic variants of small effect. This complicates genetic mapping of growth and body mass. Experimental intercrosses between individuals from divergent populations allows us to map naturally occurring genetic variants for selected traits, such as body mass by linkage mapping. By simultaneously measuring traits and intermediary molecular phenotypes, such as gene expression, one can use integrative genomics to search for potential causative genes. In this study, we use linkage mapping approach to map growth traits (N = 471) and liver gene expression (N = 130) in an advanced intercross of wild Red Junglefowl and domestic White Leghorn layer chickens. We find 16 loci for growth traits, and 1463 loci for liver gene expression, as measured by microarrays. Of these, the genes TRAK1, OSBPL8, YEATS4, CEP55, and PIP4K2B are identified as strong candidates for growth loci in the chicken. We also show a high degree of sex-specific gene-regulation, with almost every gene expression locus exhibiting sex-interactions. Finally, several trans-regulatory hotspots were found, one of which coincides with a major growth locus. These findings not only serve to identify several strong candidates affecting growth, but also show how sex-specificity and local gene-regulation affect growth regulation in the chicken. The online version of this article (10.1186/s12864-018-4441-3) contains supplementary material, which is available to authorized users.
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