The effects of housing density on mouse thermal physiology depend on sex and ambient temperature.
The effects of housing density on mouse thermal physiology depend on sex and ambient temperature.
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DOI：
10.1016/j.molmet.2021.101332
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发表时间：
2021-11
影响因子：
8.1
通讯作者：
Reitman ML
中科院分区：
文献类型：
--
作者：
Škop V;Xiao C;Liu N;Gavrilova O;Reitman ML
为了提高对小鼠能量稳态及其对人类的适用性的理解，我们定量了住房密度对两性小鼠热生理的影响。将同窝野生型和Brs 3缺失小鼠单独或分组（每笼3只）饲养，并通过间接量热法进行研究，连续测量核心体温、能量消耗、体力活动和食物摄入。在23 °C下，低于热中性，与分组饲养的对照组相比，单独饲养的雄性具有较低的体温和不变的代谢率。相比之下，单独圈养的雌性动物通过增加代谢率保持与组群圈养对照动物相似的体温。随着环境温度降低到27 °C以下，只有分组饲养的小鼠降低了它们的热导率，这可能是由于蜷缩，从而干扰了Scholander描述的能量消耗与环境温度的关系。在炎热的环境（35 °C）中，单笼小鼠的热应激较少。禁食后，单笼小鼠的体温降低幅度更大，雄性小鼠具有相似持续时间的更多麻痹发作，雌性小鼠具有相似持续时间更长的麻痹发作次数。定性地，饲养密度对Brs 3-null小鼠的热生理学的影响通常模仿对照中的影响。单室比群室更灵敏地检测热生理表型。单一住房增加了热量损失，并放大了禁食或寒冷环境的影响。雄性和雌性小鼠利用不同的体温调节策略，以应对单一住房。·改变住房密度会改变热生理和代谢率。·单独圈养的小鼠更容易受到禁食和低温的影响。·单个外壳比组外壳更灵敏，可检测热表型。·某些热生理学原理被集体居住所掩盖。雄性和雌性小鼠对单一住房的反应不同。
To improve understanding of mouse energy homeostasis and its applicability to humans, we quantitated the effects of housing density on mouse thermal physiology in both sexes. Littermate wild type and Brs3-null mice were single- or group- (three per cage) housed and studied by indirect calorimetry with continuous measurement of core body temperature, energy expenditure, physical activity, and food intake. At 23 °C, below thermoneutrality, single-housed males had a lower body temperature and unchanged metabolic rate compared to group-housed controls. In contrast, single-housed females maintained a similar body temperature to group-housed controls by increasing their metabolic rate. With decreasing ambient temperature below 27 °C, only group-housed mice decreased their heat conductance, likely due to huddling, thus interfering with the energy expenditure vs ambient temperature relationship described by Scholander. In a hot environment (35 °C), the single-housed mice were less heat stressed. Upon fasting, single-housed mice had larger reductions in body temperature, with male mice having more torpor episodes of similar duration and female mice having a similar number of torpor episodes that lasted longer. Qualitatively, the effects of housing density on thermal physiology of Brs3-null mice generally mimicked the effects in controls. Single housing is more sensitive than group housing for detecting thermal physiology phenotypes. Single housing increases heat loss and amplifies the effects of fasting or a cold environment. Male and female mice utilize different thermoregulatory strategies to respond to single housing. • Changing housing density changes thermal physiology and metabolic rate. • Singly housed mice are more affected by fasting and by cold temperatures. • Single housing is more sensitive than group housing for detecting thermal phenotypes. • Certain principles of thermal physiology are masked by group housing. • Male and female mice respond differently to single housing.
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影响因子：
64.8
作者：
通讯作者：
--
DOI：
10.1093/oso/9780198830399.001.0001
发表时间：
2019-01-01
期刊：
MEASURING METABOLIC RATES: A MANUAL FOR SCIENTISTS, 2ND EDITION
影响因子：
--
作者：
Lighton, J. R. B.
通讯作者：
Lighton, J. R. B.
影响因子：
2.9
作者：
BATCHELDER, P;KINNEY, RO;LYNCH, CB
通讯作者：
LYNCH, CB
影响因子：
2.9
作者：
Hunt, C;Hambly, C
通讯作者：
Hambly, C
DOI：
10.1152/ajpendo.00615.2013
发表时间：
2014-03-01
影响因子：
5.1
作者：
Lateef, Dalya M.;Abreu-Vieira, Gustavo;Reitman, Marc L.
通讯作者：
Reitman, Marc L.