A Label-Free Electrical Impedance Spectroscopy for Detection of Clusters of Extracellular Vesicles Based on Their Unique Dielectric Properties.
A Label-Free Electrical Impedance Spectroscopy for Detection of Clusters of Extracellular Vesicles Based on Their Unique Dielectric Properties.
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无标记的电阻抗光谱，用于根据其独特的介电特性检测细胞外囊泡的簇。
DOI：
10.3390/bios12020104
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发表时间：
2022-02-09
期刊：
影响因子：
--
通讯作者：
Esfandiari L
中科院分区：
文献类型：
--
作者：
Zhang Y;Murakami K;Borra VJ;Ozen MO;Demirci U;Nakamura T;Esfandiari L
细胞外囊泡（EV）作为重要的循环生物标志物已经获得了相当大的关注，因为它们的结构和组成类似于原始细胞。研究EV的生物化学和生物物理特性对于将其映射到其亲本细胞并更好地理解其功能具有重要意义。在这项研究中，一种新的频率相关的阻抗测量系统已被开发来表征电动汽车的基础上，其独特的介电特性。该系统由基于绝缘体的介电泳（iDEP）设备来捕获和包裹一簇囊泡，然后利用电阻抗谱（EIS）在宽频谱下测量它们的阻抗，旨在分析它们的膜和细胞溶质电荷依赖性内容物。EIS最初用于检测具有不同生物化学组成的纳米尺寸囊泡，包括用不同脂质组成合成的脂质体，以及具有相似生物物理性质但不同生物化学性质的EV和脂蛋白。此外，对来源于相同亲本细胞但用不同培养条件处理的EV进行表征，以研究阻抗变化与促炎反应方面的生化特性和功能的相关性。该系统还显示出区分来自不同细胞来源的EV以及来自相同细胞来源的大小分选的EV的能力。这种概念验证方法是利用EIS作为无标记，非侵入性和快速传感器来检测和表征未来致病性EV和其他纳米囊泡的第一步。
Extracellular vesicles (EVs) have gained considerable attention as vital circulating biomarkers since their structure and composition resemble the originating cells. The investigation of EVs’ biochemical and biophysical properties is of great importance to map them to their parental cells and to better understand their functionalities. In this study, a novel frequency-dependent impedance measurement system has been developed to characterize EVs based on their unique dielectric properties. The system is composed of an insulator-based dielectrophoretic (iDEP) device to entrap and immobilize a cluster of vesicles followed by utilizing electrical impedance spectroscopy (EIS) to measure their impedance at a wide frequency spectrum, aiming to analyze both their membrane and cytosolic charge-dependent contents. The EIS was initially utilized to detect nano-size vesicles with different biochemical compositions, including liposomes synthesized with different lipid compositions, as well as EVs and lipoproteins with similar biophysical properties but dissimilar biochemical properties. Moreover, EVs derived from the same parental cells but treated with different culture conditions were characterized to investigate the correlation of impedance changes with biochemical properties and functionality in terms of pro-inflammatory responses. The system also showed the ability to discriminate between EVs derived from different cellular origins as well as among size-sorted EVs harbored from the same cellular origin. This proof-of-concept approach is the first step towards utilizing EIS as a label-free, non-invasive, and rapid sensor for detection and characterization of pathogenic EVs and other nanovesicles in the future.
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DOI：
10.1073/pnas.1521230113
发表时间：
2016-02-23
影响因子：
11.1
作者：
Kowal, Joanna;Arras, Guillaume;Thery, Clotilde
通讯作者：
Thery, Clotilde
影响因子：
2.7
作者：
Cho, Younghak;Kim, Hyun Soo;Han, Arum
通讯作者：
Han, Arum
GW4869用GW4869的外泌体产生封锁会抑制败血症引起的炎症和心脏功能障碍。
DOI：
10.1016/j.bbadis.2015.08.010
发表时间：
2015-11
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Essandoh K;Yang L;Wang X;Huang W;Qin D;Hao J;Wang Y;Zingarelli B;Peng T;Fan GC
通讯作者：
Fan GC
影响因子：
6.2
作者：
McGrath, J. S.;Honrado, C.;Swami, N. S.
通讯作者：
Swami, N. S.
影响因子：
4.6
作者：
Lee YS;Kim SY;Ko E;Lee JH;Yi HS;Yoo YJ;Je J;Suh SJ;Jung YK;Kim JH;Seo YS;Yim HJ;Jeong WI;Yeon JE;Um SH;Byun KS
通讯作者：
Byun KS