A telomerase subunit homolog La protein from Trypanosoma brucei plays an essential role in ribosomal biogenesis
A telomerase subunit homolog La protein from Trypanosoma brucei plays an essential role in ribosomal biogenesis
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来自布氏锥虫的端粒酶亚基同源 La 蛋白在核糖体生物合成中发挥重要作用
DOI：
10.1111/febs.14853
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发表时间：
2019-08
期刊：
影响因子：
5.4
通讯作者：
Tu Xiaoming
中科院分区：
文献类型：
--
作者：
Shan Fangzhen;Mei Song;Zhang Jiahai;Zhang Xuecheng;Xu Chao;Liao Shanhui;Tu Xiaoming
自身抗原La蛋白是端粒酶的重要组成部分，是一种主要的核磷蛋白。La蛋白作为端粒酶亚单位，与端粒酶核糖核蛋白结合，影响端粒长度。在逆转录过程中，La蛋白刺激端粒酶的酶活性，增加端粒酶的重复加成持续合成能力。作为核磷蛋白，La蛋白结合新生RNA聚合酶III转录物的富含3′ poly（U）的元件，以促进其正确折叠和成熟。本研究从布氏锥虫（Trypanosoma brucei，T. brucei）。我们发现TbLa与T.布鲁塞。在TbLa蛋白与（P34/P37）/L5/SA的相互作用中，TbLa蛋白的RNA识别基序（RRM）结构域对该过程起主要作用。确定了TbLa RRM畴的溶液结构。NMR化学位移扰动揭示了TbLa RRM结构域的带正电荷的RNA结合口袋负责其与核糖体和核糖体相关蛋白P37/L5/SA的相互作用。TbLa的缺失影响了5SrRNA的成熟和核糖体的组装，提示TbLa蛋白可能在T.布鲁塞。总之，我们的研究结果为更好地理解TbLa和RRM结构域在T.布鲁塞。
The autoantigen La protein is an important component of telomerase and a predominantly nuclear phosphoprotein. As a telomerase subunit, La protein associates with the telomerase ribonucleoprotein and influences telomere length. In the reverse transcription, La protein stimulates enzymatic activity and increases repeated addition processivity of telomerase. As nuclear phosphoprotein, La protein binds the 3′ poly(U)‐rich elements of nascent RNA polymerase III transcripts to facilitate its correct folding and maturation. In this work, we identified a La protein homolog (TbLa) from Trypanosoma brucei (T. brucei). We revealed that TbLa interacts with ribosome‐associated protein P34/P37, 40S ribosomal protein SA, and 60S ribosomal subunit L5 in T. brucei. In the interactions between TbLa protein and (P34/P37)/L5/SA, RNA recognition motif (RRM) domain of TbLa was indicated to make the major contribution to the processes. We determined the solution structure of TbLa RRM domain. NMR chemical shift perturbations revealed that the positively charged RNA‐binding pocket of TbLa RRM domain is responsible for its interaction with ribosomal and ribosome‐associated proteins P37/L5/SA. Furthermore, depletion of TbLa affected the maturation process of 5S rRNA and ribosomal assembly, suggesting TbLa protein might play a significant role in the ribosomal biogenesis pathway in T. brucei. Taken together, our results provide a novel insight and structural basis for better understanding the roles of TbLa and RRM domain in ribosomal biogenesis in T. brucei.
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布氏锥虫 TbCentrin4 的溶液结构及其与 Ca2 和其他中心蛋白的相互作用
影响因子：
4.1
作者：
Fangzhen Shan;Kaiqin Ye;Jiahai Zhang;Shanhui Liao;Xuecheng Zhang;Chao Xu;Xiaoming Tu
通讯作者：
Xiaoming Tu
影响因子：
--
作者：
Khan Umaer;Martín Ciganda;N. Williams
通讯作者：
Khan Umaer;Martín Ciganda;N. Williams
DOI：
10.1083/jcb.153.4.f13
发表时间：
2001-05-14
期刊：
The Journal of cell biology
影响因子：
--
作者：
Maraia RJ
通讯作者：
Maraia RJ
影响因子：
--
作者：
Heise T;Kota V;Brock A;Morris AB;Rodriguez RM;Zierk AW;Howe PH;Sommer G
通讯作者：
Sommer G
影响因子：
7.3
作者：
Ciganda, Martin;Williams, Noreen
通讯作者：
Williams, Noreen