The scavenging of reactive oxygen species and the potential for cell protection by functionalized fullerene materials.
The scavenging of reactive oxygen species and the potential for cell protection by functionalized fullerene materials.
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DOI：
10.1016/j.biomaterials.2008.09.061
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发表时间：
2009-03
期刊：
影响因子：
14
通讯作者：
Chen C
中科院分区：
文献类型：
--
作者：
Yin JJ;Lao F;Fu PP;Wamer WG;Zhao Y;Wang PC;Qiu Y;Sun B;Xing G;Dong J;Liang XJ;Chen C
我们证明了三种不同类型的水溶性富勒烯材料可以拦截所有主要的生理相关的活性氧。C60（C（COOH）2）2、C60（OH）22和Gd@C82（OH）22可保护细胞免受H2 O2诱导的氧化损伤，稳定线粒体膜电位并减少细胞内ROS产生，其相对效力如下：Gd@C82（OH）22 ≥ C60（OH）22> C60（C（COOH）2）2。与它们的细胞保护能力一致，这些衍生物可以抑制稳定的2，2-二苯基-1-苦肼基自由基（DPPH·）和活性氧（ROS）超氧阴离子自由基（O2· −）、单线态氧和羟基自由基（HO·），并且还可以在体外有效地抑制脂质过氧化。所观察到的自由基清除能力的差异支持的假设，这两个化学性质，如表面化学诱导的电子亲和力的差异，和物理性质，如聚集度，影响功能化富勒烯的生物和生物医学活性。这是首次报道不同类型的富勒烯衍生物可以抑制所有生理相关的ROS。氧化应激和损伤在许多疾病的病因学和进展中的作用表明，这些富勒烯衍生物可能是有价值的体内细胞保护剂和治疗剂。
We demonstrated that three different types of water-soluble fullerenes materials can intercept all of the major physiologically relevant ROS. C60(C(COOH)2)2, C60(OH)22, and Gd@C82(OH)22 can protect cells against H2O2-induced oxidative damage, stabilize the mitochondrial membrane potential and reduce intracellular ROS production with the following relative potencies: Gd@C82(OH)22 ≥ C60(OH)22 > C60(C(COOH)2)2. Consistent with their cytoprotective abilities, these derivatives can scavenge the stable 2,2-diphenyl-1-picryhydrazyl radical (DPPH•), and the reactive oxygen species (ROS) superoxide radical anion (O2• −), singlet oxygen, and hydroxyl radical (HO•), and can also efficiently inhibit lipid peroxidation in vitro. The observed differences in free radical scavenging capabilities support the hypothesis that both chemical properties, such as surface chemistry induced differences in electron affinity, and physical properties, such as degree of aggregation, influence the biological and biomedical activities of functionalized fullerenes. This represents the first report that different types of fullerene derivatives can scavenge all physiologically relevant ROS. The role of oxidative stress and damage in the etiology and progression of many diseases suggests that these fullerene derivatives may be valuable in vivo cytoprotective and therapeutic agents.
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影响因子：
2.6
作者：
Lai, HS;Chen, WJ;Chiang, LY
通讯作者：
Chiang, LY
DOI：
10.1016/0005-2736(86)90124-0
发表时间：
1986-01-29
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
KUSUMI, A;SUBCZYNSKI, WK;MERKLE, H
通讯作者：
MERKLE, H
影响因子：
0.9
作者：
Chueh, SC;Lai, MK;Chen, SC
通讯作者：
Chen, SC
影响因子：
0.9
作者：
Kopylov, VB;Gavronskaya, YY
通讯作者：
Gavronskaya, YY
影响因子：
14
作者：
Markovic, Zoran;Todorovic-Markovic, Biljana;Trajkovic, Vladimir
通讯作者：
Trajkovic, Vladimir