Genomic structure, tissue expression and single nucleotide polymorphisms of lipoprotein lipase and hepatic lipase genes in Chinese perch
Genomic structure, tissue expression and single nucleotide polymorphisms of lipoprotein lipase and hepatic lipase genes in Chinese perch
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中华鲈鱼脂蛋白脂肪酶和肝脂肪酶基因的基因组结构、组织表达及单核苷酸多态性
DOI：
10.1111/anu.12292
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发表时间：
2016-08
影响因子：
3.5
通讯作者：
Tao Yaxiong
中科院分区：
文献类型：
--
作者：
Sun Jian;Wen Zhenyong;Shen Dan;Tao Yaxiong
鲈鱼是一种专性食鱼性鱼类，可作为研究脂蛋白脂酶（Lpl）和肝脂酶（Hl）基因多样性的理想模式系统。本研究对鲈鱼Lpl和Hl基因的基因组结构、组织表达和单核苷酸多态性进行了研究。与其他脊椎动物的结果一致，鲈鱼Lpl基因由10个外显子和9个内含子组成，Hl基因由9个外显子和8个内含子组成。H1基因启动子区与鲈鱼Lpl基因启动子区同源性不高。鲈鱼Lpl基因mRNA在脂肪组织中的表达量最高，其次是肝脏、脑、肠和肌肉，脾脏中的表达量最低，而Hl基因几乎只在肝脏中表达。这些结果表明，鲈鱼Lpl和Hl可能是由祖先基因的早期复制衍生的。通过对每组130条鱼的Lpl和Hl基因进行直接测序，检测了死亡猎物鱼的饲养者和非饲养者中Lpl和Hl基因的多态性。在Lpl基因中检测到A1220T、G1221T和C1224G三个SNP位点，而在Hl基因中未检测到SNP位点。Lpl基因2型与鱼对死鱼的接受性显著相关。
Chinese perch, an obligate piscivorous fish, could serve as an excellent model system for studies on the diversification of lipoprotein lipase (Lpl) and hepatic lipase (Hl) genes. In this study, we characterized the genomic structure, tissue expression and single nucleotide polymorphisms of Lpl and Hl genes in Chinese perch (Siniperca chuatsi). Consistent with findings in other vertebrates, Chinese perch Lpl gene consisted of ten exons and nine introns, and Hl gene consisted of nine exons and eight introns. The promoter region of Hl gene was not highly homologous to that of Lpl gene in Chinese perch. The mRNA expression of Lpl in Chinese perch was the highest in adipose tissue, followed by liver, brain, intestine and muscle, and the lowest in spleen, whereas Hl was almost exclusively expressed in liver. These results suggested that Chinese perch Lpl and Hl might be derived from an early duplication of an ancestral gene. Polymorphisms of Lpl and Hl genes were examined in feeders and non-feeders of dead prey fish by direct sequencing of these genes in 130 fish from each group. Three SNPs (A1220T, G1221T and C1224G) were identified in Lpl gene, whereas none was identified in Hl gene. Diplotype 2 in Lpl gene was significantly correlated with acceptance of dead prey fish.
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影响因子：
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作者：
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通讯作者：
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