Postnatal meningeal CSF transport is primarily mediated by the arachnoid and pia maters and is not altered after intraventricular hemorrhage-posthemorrhagic hydrocephalus.
Postnatal meningeal CSF transport is primarily mediated by the arachnoid and pia maters and is not altered after intraventricular hemorrhage-posthemorrhagic hydrocephalus.
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DOI：
10.1186/s12987-023-00503-7
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发表时间：
2024-01-08
影响因子：
7.3
通讯作者：
Strahle, Jennifer M.
中科院分区：
文献类型：
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作者：
Pan, Shelei;Koleske, Joshua P.;Koller, Gretchen M.;Halupnik, Grace L.;Alli, Abdul-Haq O.;Koneru, Shriya;DeFreitas, Dakota;Ramagiri, Sruthi;Strahle, Jennifer M.
长期以来，CSF被认为在蛛网膜下腔循环，蛛网膜下腔位于脑膜的蛛网膜和软脑膜之间。CSF如何与发育中的出生后脑膜的细胞成分相互作用，包括脑表面脑膜和颅内脑膜的硬脑膜、蛛网膜和软脑膜，在其最终从颅骨和脊柱流出之前，尚不清楚。在这里，我们的特点小和大的CSF溶质分布模式沿着在新生啮齿动物的颅内和表面脑膜，并比较我们的研究结果，脑膜CSF溶质分布在啮齿动物模型的脑室内血肿出血后脑积水。我们还研究了CSF溶质与脉络膜组织及其软脑膜内陷到侧脑室、第三和第四脑室脉络丛的相互作用。将在aCSF中构成的1.9-nm金纳米颗粒、15-nm金纳米颗粒或3 kDa红葡聚糖四甲基罗丹明注入P7大鼠的右侧脑室以追踪CSF循环。1.9-nm金纳米颗粒和红色葡聚糖四甲基罗丹明注射后10分钟和15-nm金纳米颗粒注射后4小时，处死动物并收获脑用于组织学分析以鉴定颅和脊膜以及脉络丛中的CSF示踪剂定位。还评价了硬脊膜和软脑膜（蛛网膜和软脑膜）整体标本。在新生啮齿动物中，与蛛网膜和软脑膜相比，硬脑膜中的CSF示踪剂分布显著较少。小和大的CSF示踪剂都被颅内运输到中脑周围池和脉络膜的蛛网膜和软脑膜，但不是大脑镰。CSF示踪剂在脊膜中遵循类似的分布模式。脉络丛上皮细胞顶面有大量CSF示踪剂分布，基底面沿着有少量CSF示踪剂分布。对照组与脑室内出血后脑积水（PHH）啮齿动物颅内脑膜中的示踪剂强度无显著差异，表明PHH环境中保留了脑膜运输。脑膜对CSF示踪剂的处理差异表明，在发育中的大脑中，蛛网膜和硬脑膜之间对CSF处理有不同的作用。类似地，顶脉络丛CSF处理与腔脉络丛CSF处理的差异可以提供对CSF-脉络丛边界处的颗粒大小依赖性CSF运输的了解。在线版本包含补充材料，可通过10.1186/s12987-023-00503-7获得。
CSF has long been accepted to circulate throughout the subarachnoid space, which lies between the arachnoid and pia maters of the meninges. How the CSF interacts with the cellular components of the developing postnatal meninges including the dura, arachnoid, and pia of both the meninges at the surface of the brain and the intracranial meninges, prior to its eventual efflux from the cranium and spine, is less understood. Here, we characterize small and large CSF solute distribution patterns along the intracranial and surface meninges in neonatal rodents and compare our findings to meningeal CSF solute distribution in a rodent model of intraventricular hemorrhage-posthemorrhagic hydrocephalus. We also examine CSF solute interactions with the tela choroidea and its pial invaginations into the choroid plexuses of the lateral, third, and fourth ventricles. 1.9-nm gold nanoparticles, 15-nm gold nanoparticles, or 3 kDa Red Dextran Tetramethylrhodamine constituted in aCSF were infused into the right lateral ventricle of P7 rats to track CSF circulation. 10 min post-1.9-nm gold nanoparticle and Red Dextran Tetramethylrhodamine injection and 4 h post-15-nm gold nanoparticle injection, animals were sacrificed and brains harvested for histologic analysis to identify CSF tracer localization in the cranial and spine meninges and choroid plexus. Spinal dura and leptomeninges (arachnoid and pia) wholemounts were also evaluated. There was significantly less CSF tracer distribution in the dura compared to the arachnoid and pia maters in neonatal rodents. Both small and large CSF tracers were transported intracranially to the arachnoid and pia mater of the perimesencephalic cisterns and tela choroidea, but not the falx cerebri. CSF tracers followed a similar distribution pattern in the spinal meninges. In the choroid plexus, there was large CSF tracer distribution in the apical surface of epithelial cells, and small CSF tracer along the basolateral surface. There were no significant differences in tracer intensity in the intracranial meninges of control vs. intraventricular hemorrhage-posthemorrhagic hydrocephalus (PHH) rodents, indicating preserved meningeal transport in the setting of PHH. Differential CSF tracer handling by the meninges suggests that there are distinct roles for CSF handling between the arachnoid-pia and dura maters in the developing brain. Similarly, differences in apical vs. luminal choroid plexus CSF handling may provide insight into particle-size dependent CSF transport at the CSF-choroid plexus border. The online version contains supplementary material available at 10.1186/s12987-023-00503-7.
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影响因子：
64.8
作者：
Ahn, Ji Hoon;Cho, Hyunsoo;Koh, Gou Young
通讯作者：
Koh, Gou Young
影响因子：
11.8
作者：
DeSisto J;O'Rourke R;Jones HE;Pawlikowski B;Malek AD;Bonney S;Guimiot F;Jones KL;Siegenthaler JA
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Siegenthaler JA
影响因子：
14.5
作者：
Botfield, Hannah;Gonzalez, Ana Maria;Logan, Ann
通讯作者：
Logan, Ann
DOI：
10.1152/ajpcell.2000.279.6.c1685
发表时间：
2000-12-01
影响因子：
5.5
作者：
Ellis, DZ;Nathanson, JA;Sweadner, KJ
通讯作者：
Sweadner, KJ
影响因子：
7.3
作者：
通讯作者：
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