Translation initiation site of the coat protein messenger ribonucleic acid of the cowpea strain of tobacco mosaic virus.
Translation initiation site of the coat protein messenger ribonucleic acid of the cowpea strain of tobacco mosaic virus.
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烟草花叶病毒豇豆株外壳蛋白信使核糖核酸的翻译起始位点。
DOI：
10.1021/bi00566a040
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发表时间：
1980
期刊：
影响因子：
2.9
通讯作者：
ClarkJr,JM
中科院分区：
文献类型：
--
作者：
Browning,KS;ClarkJr,JM
材料与方法材料。烟草花叶病毒的豌豆品系由M.Zaitlin博士慷慨提供。用于蛋白质合成分析的15种14C标记氨基酸的混合物从Am-ersham公司购买。无载体的[32P]P；来自新英格兰核公司。[7-32P]ATP由[32*]P；按Johnson&Walseth(1979)的方法通过使用从Boehringer Mannheim购买的酶来制备。M7G(5‘)PPP(5’)G、m7G(5/)PPP(5/)GM和核酸酶P]购自PL BioChemical，Inc.核糖核酸酶PhyM由H.Donis-Keller博士慷慨提供。小牛肠磷酸酶购自Boehringer-Mannheim。T4多核苷酸激酶(22000单位/毫升)是DR赠送的礼物。OC乌伦贝克。用于“漂移斑点”分析的DEAE-纤维素薄层层析板购自Brinkman Co.和Analtech。HEPES Buffer购自CalBioChem-Behring公司，斯巴霉素购自Upjohn公司，其余试剂均购自Sigma Chemical Co.制备CC-TMV的S RNA。从侵染CC-TMV的菜豆叶片中分离到CC-TMV小病毒粒子的浓缩制剂。Bountiful)，作者Bruning等人(1976)。从该制剂中提取CC-TMV的RNA(Beachy&Zaitlin，1977)，然后通过凝胶电泳和电洗脱将CC-TMV S-RNA从其他RNA中分离出来，然后酚提取和乙醇沉淀(得率=50 mg CC-TMV S-RNA/200g病叶)。甘蓝型烟草花叶病毒S核糖核酸的制备用Richards等人(1978)的方法从CC-TMV的S核糖核酸中去除了潜在的S末端的“帽”基团，并重新.
Materials and Methods Materials. The cowpea strain ofTMV was generously provided by Dr. M. Zaitlin. A mixture of 15 14C-labeled amino acids for protein synthesis assays was purchased from Am-ersham Corp. Carrier-free [32P] P; was from New England Nuclear Corp.[7-32P] ATP was prepared from [32**** P] P; by the method of Johnson & Walseth (1979) by using enzymes purchased from Boehringer Mannheim. m7G (5') ppp (5') G, m7G (5/) ppp (5/) Gm, and nuclease P] were obtained from PL Biochemicals, Inc. RNase A, RNase Tb and RNase U2 were purchased from Calbiochem-Behring Corp. Ribonuclease PhyM was generously provided by Dr. H. Donis-Keller. Calf intestinal phosphatase was purchased from Boehringer-Mannheim. T4 polynucleotide kinase (22000 units/mL) was a gift fromDr. OC Uhlenbeck. DEAE-cellulose thin-layer chromatography plates for “wandering spot” analyses were purchased from Brinkman Co. and Analtech. Inc. Hepes buffer was purchased from Calbiochem-Behring Corp. Sparsomycin was obtained from the Upjohn Co. All other reagents were as received from Sigma Chemical Co. Preparation of the S-RNA of Cc-TMV. An enriched preparation of the small virion of Cc-TMV was isolated from Cc-TMV infected bean leaves (Phaseolus vulgaris, cv. Bountiful) by the method of Bruening et al.(1976). Cc-TMV RNAs were extracted (Beachy & Zaitlin, 1977) from this preparation and then the Cc-TMV S-RNA was resolved from the other RNAs by gel electrophoresis and electroelutionprior to phenol extraction and alcohol precipitation (yield= 50 Mg of Cc-TMV S-RNA/200 g of infected leaves). Preparation of the S-{5’-nP] RNA of Cc-TMV. Potential S'-terminal “cap” groups were removed from the S-RNA of Cc-TMV by the method of Richards et al.(1978), and re-
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烟草花叶病毒番茄和豇豆菌株 RNA 上杆组装的起始位点。
影响因子：
3.7
作者：
M. Fukuda;Y. Okada;Y. Otsuki;I. Takebe
通讯作者：
I. Takebe
烟草花叶病毒RNA病毒重组起点的定位及其与稳定片段的关系
影响因子：
3.5
作者：
D. Zimmern;T. Wilson
通讯作者：
T. Wilson
影响因子：
3.7
作者：
BEACHY, RN;ZAITLIN, M;ISRAEL, HW
通讯作者：
ISRAEL, HW
感染 TMV 豇豆菌株的豆子中出现短颗粒。
影响因子：
3.7
作者：
Thomas J. V. Higgins;Peter B. Goodwin;P. Whitfeld
通讯作者：
P. Whitfeld
感染 TMV 豇豆菌株的豆子中出现短颗粒。
影响因子：
3.7
作者：
P. Whitfeld;T. Higgins
通讯作者：
T. Higgins