Impaired experience-dependent maternal care in presynaptic active zone protein CAST-deficient dams
Impaired experience-dependent maternal care in presynaptic active zone protein CAST-deficient dams
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突触前活性区蛋白 CAST 缺陷母鼠的经验依赖性母婴护理受损
DOI：
10.1038/s41598-020-62072-1
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发表时间：
2020
影响因子：
4.6
通讯作者：
T.*
中科院分区：
文献类型：
--
作者：
Hagiwara;A.;Sugiyama;N.;Ohtsuka;T.*
尽管社会学研究肯定了父母照顾对后代生存的重要性，但虐待——包括忽视儿童——在许多国家仍然普遍存在。众所周知，儿童忽视会影响儿童发展，但对母亲忽视的原因却知之甚少。在这里，我们发现CAST（一种突触前释放机制蛋白）缺失突变的雌性小鼠在初产时断奶率显著降低，在多产时恢复率显著降低。事实上，在哺育过程中，初产和未产CAST敲除（KO）小鼠表现出比对照组小鼠更少的蹲伏时间和更大的移动距离。与预期相反，尽管垂体后叶大细胞神经元末梢表达CAST，但CAST KO小鼠血浆催产素（OXT）并未显著降低。我们进一步发现，与对照组小鼠相比，CAST KO小鼠在培养时喝的水明显减少，并且在怀孕期间对蔗糖有更大的偏好。我们认为突触前释放机制蛋白的缺乏损害了一些母性行为的促进性，这可以通过经验和学习来弥补。
Although sociological studies affirm the importance of parental care in the survival of offspring, maltreatment—including child neglect—remains prevalent in many countries. While child neglect is well known to affect child development, the causes of maternal neglect are poorly understood. Here, we found that female mice with a deletion mutation of CAST (a presynaptic release-machinery protein) showed significantly reduced weaning rate when primiparous and a recovered rate when multiparous. Indeed, when nurturing, primiparous and nulliparous CAST knock out (KO) mice exhibited less crouching time than control mice and moved greater distances. Contrary to expectations, plasma oxytocin (OXT) was not significantly reduced in CAST KO mice even though terminals of magnocellular neurons in the posterior pituitary expressed CAST. We further found that compared with control mice, CAST KO mice drank significantly less water when nurturing and had a greater preference for sucrose during pregnancy. We suggest that deficiency in presynaptic release-machinery protein impairs the facilitation of some maternal behaviours, which can be compensated for by experience and learning.
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影响因子：
2.5
作者：
Hagiwara, A;Fukazawa, Y;Shigemoto, R
通讯作者：
Shigemoto, R
影响因子：
15.9
作者：
T. Fisher;C. Bourque
通讯作者：
T. Fisher;C. Bourque
影响因子：
5.3
作者：
Dieck, Susanne Tom;Specht, Dana;Moser, Tobias
通讯作者：
Moser, Tobias
关于哺乳动物母性动机的新理论和实验方法
影响因子：
8.2
作者：
D. Olazábal;Mariana Pereira;D. Agrati;Annabel Ferreira;A. Fleming;G. González;F. Lévy;A. Lucion;J. Morrell;M. Numan;N. Uriarte
通讯作者：
N. Uriarte
DOI：
10.1073/pnas.0505312102
发表时间：
2005-11-01
影响因子：
11.1
作者：
Takayanagi, Y;Yoshida, M;Nishimori, K
通讯作者：
Nishimori, K