Cloning, sequencing, and tissue-dependent expression of flavin-containing monooxygenase (FMO) 1 and FMO3 in the dog.
Cloning, sequencing, and tissue-dependent expression of flavin-containing monooxygenase (FMO) 1 and FMO3 in the dog.
复制标题
狗体内含黄素单加氧酶 (FMO) 1 和 FMO3 的克隆、测序和组织依赖性表达。
DOI：
10.1124/dmd.30.2.119
复制
发表时间：
2002
期刊：
影响因子：
--
通讯作者：
E. Benoît
中科院分区：
文献类型：
--
作者：
V. Lattard;C. Longin‐Sauvageon;J. Lachuer;P. Delatour;E. Benoît
含有黄素的单加氧酶(FMOS)在狗的肝微粒体中表达，表明其具有较高的他巴唑S氧化酶活性。在狗肝微粒体催化下，表观V(Max)和K(M)分别为6.3nmol/min/mg和14微米。在丙咪胺存在下，该反应被高度抑制(73%)，但三甲胺对该反应的影响也很弱，这表明不同的亚型参与了该反应。通过逆转录-聚合酶链式反应和5‘/3’末端延伸获得犬FMO1和FMO3基因序列。犬FMO1和FMO3的cDNA编码532个氨基酸的蛋白质，含有NADPH和FAD结合位点。犬FMO1的氨基酸序列与人、兔和猪的FMO1的同源性分别为88%、86%和89%。犬FMO3的氨基酸序列与人、兔和大鼠的FMO3的同源性分别为83%、84%和82%。狗FMO1和狗FMO3只有56%的同源性。SDS-聚丙烯酰胺凝胶电泳法和免疫印迹法检测到Fmo1和Fmo3重组蛋白以及Fmo1和Fmo3微粒体蛋白迁移的迁移率相同(56 KDa)。Western blotting检测到狗FMO1和FMO3在肝和肺的微粒体中表达，而在肾微粒体中未检测到。通过Northern blotting，FMO1的探针仅在肝脏和肺样本中特异性地杂交了2.6kb的转录本。FMO3的探针在肝脏和肺样本中杂交了大约3kb和4.2kb的两个转录本。
The expression of flavin-containing monooxygenases (FMOs) in dog liver microsomes was suggested by a high methimazole S-oxidase activity. When the reaction was catalyzed by dog liver microsomes, apparent V(max) and K(m) values were 6.3 nmol/min/mg and 14 microM, respectively. This reaction was highly inhibited (73%) in the presence of imipramine, but it was also weakly affected by trimethylamine, suggesting the involvement of different isoforms. The sequences of dog FMO1 and FMO3 were obtained by reverse transcription-polymerase chain reaction and 5'/3' terminal extension. The cDNAs of dog FMO1 and dog FMO3 encode proteins of 532 amino acids, which contain the NADPH- and FAD-binding sites. The dog FMO1 amino acid sequence is 88, 86, and 89% identical to sequences of human, rabbit, and pig FMO1, respectively. The dog FMO3 amino acid sequence is 83, 84, and 82% identical to sequences of human, rabbit, and rat FMO3, respectively. Dog FMO1 and dog FMO3 exhibited only 56% identities. The FMO1 and FMO3 recombinant proteins and the FMO1 and FMO3 microsomal proteins migrated with the same mobility (56 kDa), as determined in SDS-polyacrylamide gel electrophoresis and immunoblotting. By Western blotting, dog FMO1 and dog FMO3 were detected in microsomes from liver and lung but not in kidney microsomes. By Northern blotting, the probe for FMO1 specifically hybridized a 2.6-kilobase (kb) transcript in liver and lung samples only. The probe for FMO3 hybridized two transcripts of approximately 3 and 4.2 kb in the liver and lung samples.
登录
查看更多内容
肝脏和肾脏微粒体中含黄素单加氧酶 3 型表达的物种和性别差异。
DOI：
--
发表时间：
1999
期刊：
Drug metabolism and disposition: the biological fate of chemicals.
影响因子：
--
作者：
Ripp,SL;Itagaki,K;Philpot,RM;Elfarra,AA
通讯作者：
Elfarra,AA
来自成人肝脏的含黄素单加氧酶（II 型）cDNA 的分子克隆。
DOI：
10.1073/pnas.89.5.1685
发表时间：
1992
影响因子：
11.1
作者：
Lomri,N;Gu,Q;Cashman,JR
通讯作者：
Cashman,JR
兔肺含黄素单加氧酶在免疫化学和催化方面与肝酶不同。
DOI：
10.1016/s0006-291x(84)80342-3
发表时间：
1984
影响因子：
3.1
作者：
Williams,DE;Ziegler,DM;Nordin,DJ;Hale,SE;Masters,BS
通讯作者：
Masters,BS
从小鼠和猪肝微粒体中纯化含黄素单加氧酶。
DOI：
10.1016/0020-711x(84)90180-0
发表时间：
1984
期刊：
The International journal of biochemistry
影响因子：
--
作者：
Sabourin,PJ;Smyser,BP;Hodgson,E
通讯作者：
Hodgson,E
各种猪、小鼠、大鼠、兔、狗和人体组织中微粒体含黄素单加氧酶的免疫化学比较和定量。
影响因子：
3.6
作者：
Dannan,GA;Guengerich,FP
通讯作者：
Guengerich,FP