Progress toward the application of molecular force spectroscopy to DNA sequencing.
Progress toward the application of molecular force spectroscopy to DNA sequencing.
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DOI：
10.1002/elps.201200351
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发表时间：
2012-12
期刊：
影响因子：
2.9
通讯作者：
Vezenov, Dmitri
中科院分区：
文献类型：
--
作者：
Cheng, Peng;Oliver, Piercen M.;Barrett, Michael J.;Vezenov, Dmitri
DNA 测序的许多最新进展都利用了使用荧光标记寡核苷酸作为主要检测模式的单分子技术。然而，尽管基于荧光的测序仪取得了成功，但避免标记核苷酸可以大大降低测序成本。本文讨论了另一种测序方法的开发，其中可以使用单分子力谱在大规模并行规模上直接操纵未标记的 DNA。我们将宽视场光学检测技术（倏逝场激发）与两种平行施力方法（磁性或介电电泳 (DEP) 镊子）之一相结合，以在 DNA 双链特征中获得接近单碱基的灵敏度。本文将讨论这种单分子力谱技术作为基因组测序潜在技术的发展。
Many recent advances in DNA sequencing have taken advantage of single-molecule techniques using fluorescently-labeled oligonucleotides as the principle mode of detection. However, in spite of the successes of fluorescent-based sequencers, avoidance of labeled nucleotides could substantially reduce the costs of sequencing. This paper discusses the development of an alternative sequencing method in which unlabeled DNA can be manipulated directly on a massively-parallel scale using single-molecule force spectroscopy. We combine a wide-field optical detection technique (evanescent field excitation) with one of two methods of applying force in parallel, magnetic or dielectrophoretic (DEP) tweezers, to attain near single-base sensitivity in the double-stranded character of DNA. This article will discuss the developments of such a single-molecule force spectroscopy technique as a potential technology for genome sequencing.
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影响因子：
64.8
作者：
Bustamante, C;Bryant, Z;Smith, SB
通讯作者：
Smith, SB
影响因子：
7.4
作者：
Barrett, Michael J.;Oliver, Piercen M.;Cheng, Peng;Cetin, Deniz;Vezenov, Dmitri
通讯作者：
Vezenov, Dmitri
影响因子：
6.7
作者：
Oliver PM;Park JS;Vezenov D
通讯作者：
Vezenov D
影响因子：
10.8
作者：
McNally B;Singer A;Yu Z;Sun Y;Weng Z;Meller A
通讯作者：
Meller A
影响因子：
15
作者：
Cockroft, Scott L.;Chu, John;Ghadiri, M. Reza
通讯作者：
Ghadiri, M. Reza