NeuroProtect, a Candidate Formula From Traditional Chinese Medicine, Attenuates Amyloid-β and Restores Synaptic Structures in APP/PS1 Transgenic Mice.
NeuroProtect, a Candidate Formula From Traditional Chinese Medicine, Attenuates Amyloid-β and Restores Synaptic Structures in APP/PS1 Transgenic Mice.
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DOI：
10.3389/fphar.2022.850175
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发表时间：
2022
影响因子：
5.6
通讯作者：
Hua, Qian
中科院分区：
文献类型：
--
作者：
Tan, Yan;Wang, Xu;Zhang, Jiani;Zhang, Huawei;Li, Haiyan;Peng, Tiantian;Chen, Weihang;Wei, Peng;Liu, Zhaoheng;He, Fang;Li, Jiao;Ding, Haimin;Li, Na;Wang, Zhaoyang;Zhang, Zhenqiang;Hua, Qian
背景：阿尔茨海默病（AD）是痴呆症最常见的原因。新出现的数据表明，认知能力下降发生在Aβ蓄积伴突触功能障碍的情况下，这在临床前阶段开始发生。因此，症状前干预对延缓AD加工更为关键。中医药在治疗和预防痴呆症方面有着悠久的历史。研究表明三七栀子水煎剂能增强脑卒中患者的记忆功能，其主要成分三七皂苷（PNS）和栀子苷（GP）能改善实验性AD模型的记忆能力。由于草药在保护方面具有副作用少的优势，我们希望扩展NeuroProtect（NP）制剂在APP/PS1转基因小鼠中减少淀粉样蛋白-β和恢复突触结构的观察。 方法：APP/PS1转基因小鼠和它们的野生型同窝仔从4至7月龄喂养对照、NP及其组分。我们通过高尔基体染色评估突触结构，通过硫磺素-S染色分析淀粉样蛋白沉积，并通过Western blot或ELISA测量相关蛋白水平。采用Morris水迷宫和穿梭箱实验评价认知功能。 结果如下：与WT小鼠相比，APP/PS1小鼠的特征是淀粉样蛋白斑块的积累，减少突触结构的丰富性和记忆缺陷。NP阻止这些变化并改善认知缺陷。这些作用可能是由于其组分通过抑制不溶性淀粉样蛋白-β沉积和恢复突触结构而发挥的作用。 结论：这些发现揭示了在早期干预策略下NP对AD进展的有益作用，并为AD预防提供了食物补充。
Background: Alzheimer’s disease (AD) is the most common cause of dementia. The emerging data suggest that cognitive decline occurred in the setting of Aβ accumulation with synaptic dysfunction, which started to happen at preclinical stages. Then, presymptomatic intervention is more critical to postponing AD processing. Traditional Chinese medicine has a long history of treating and preventing dementia. Findings have shown that the decoction of Panax notoginseng and Gardenia jasminoides Ellis enhances memory functions in patients with stroke, and their main components, Panax notoginseng saponins (PNS) and geniposide (GP), improved memory abilities in experimental AD models. Since herbal medicine has advantages in protection with few side effects, we wish to extend observations of the NeuroProtect (NP) formulation for reducing amyloid-β and restoring synaptic structures in APP/PS1 transgenic mice. Methods: APP/PS1 transgenic mice and their wild-type littermates were fed with control, NP, and their components from 4 to 7 months of age. We assessed the synaptic structure by Golgi staining, analyzed the amyloid deposits by Thioflavin-S staining, and measured related protein levels by Western blot or ELISA. We used the Morris water maze and shuttle box test to evaluate cognitive functions. Results: Compared to WT mice, APP/PS1 mice are characterized by the accumulation of amyloid plaques, reducing synaptic structure richness and memory deficits. NP prevents these changes and ameliorates cognitive deficits. These effects may have been due to the contribution of its components by inhibition of insoluble amyloid-β deposition and restoration of synaptic structures. Conclusion: These findings reveal a beneficial effect of NP on AD progression under an early intervention strategy and provide a food supplement for AD prevention.
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影响因子：
168.9
作者：
Ballard, Clive;Gauthier, Serge;Jones, Emma
通讯作者：
Jones, Emma
影响因子：
14
作者：
Li, Song;Wu, Zhengzhi;Le, Weidong
通讯作者：
Le, Weidong
影响因子：
5.4
作者：
Hua, Qian;Qing, Xuemei;Zhu, Xiaolei
通讯作者：
Zhu, Xiaolei
影响因子：
2
作者：
Ishibashi, Kenji;Miura, Yoshiharu;Ishii, Kenji
通讯作者：
Ishii, Kenji
DOI：
10.1016/s1474-4422(18)30290-4
发表时间：
2018-09-01
期刊：
The Lancet. Neurology
影响因子：
--
作者：
Liu, Tianlong;Ding, Yi;Wen, Aidong
通讯作者：
Wen, Aidong