Micromachined optical flow cell for sensitive measurement of droplets in tubing.
Micromachined optical flow cell for sensitive measurement of droplets in tubing.
复制标题
DOI：
10.1007/s10544-018-0337-x
复制
发表时间：
2018-10-29
影响因子：
2.8
通讯作者：
Niu X
中科院分区：
文献类型：
--
作者：
Hassan SU;Nightingale AM;Niu X
这里提出了一种具有增强光学灵敏度的微加工流通池，可以对微滴进行高通量分析。当液滴流经多个串联的测量点时，可以在不停止流动的情况下充分表征液滴中的酶反应速率。由于液滴之间不存在串扰，因此流动池能够连续测量液滴流中的生化测定，因此适合用于连续的护理点诊断监测。本文介绍了该装置的设计和操作，以及通过使用氧化酶酶法准确、连续定量葡萄糖浓度进行验证。流通池是将化学和生物分析仪小型化为便携式或可穿戴设备的重要组成部分。
Here a micromachined flow cell with enhanced optical sensitivity is presented that allows high-throughput analysis of microdroplets. As a droplet flows through multiple concatenated measurement points, the rate of enzymatic reaction in the droplet can be fully characterized without stopping the flow. Since there is no cross-talk between the droplets, the flow cell is capable of continuously measuring biochemical assays in a droplet flow and thus is suitable to be used for continuous point-of-care diagnostics monitoring. This paper describes the design and operation of the device and its validation by application to the accurate and continuous quantification of glucose concentrations using an oxidase enzymatic assay. The flow cell forms an important component in the miniaturization of chemical and bio analyzers into portable or wearable devices.
登录
查看更多内容
影响因子：
3.4
作者：
Hassan SU;Nightingale AM;Niu X
通讯作者：
Niu X
影响因子：
7.4
作者：
Gielen, Fabrice;van Vliet, Liisa;Koprowski, Bartosz T.;Devenish, Sean R. A.;Fischlechner, Martin;Edel, Joshua B.;Niu, Xize;deMello, Andrew J.;Hollfelder, Florian
通讯作者：
Hollfelder, Florian
影响因子：
6.1
作者：
Rushworth, Cathy M.;Jones, Gareth;Morgan, Hywel
通讯作者：
Morgan, Hywel
影响因子：
5
作者：
SUGIURA, M;HIRANO, K
通讯作者：
HIRANO, K
影响因子：
6.1
作者：
Sista R;Hua Z;Thwar P;Sudarsan A;Srinivasan V;Eckhardt A;Pollack M;Pamula V
通讯作者：
Pamula V