Embryonic stem cells express growth hormone receptors: regulation by retinoic acid.
Embryonic stem cells express growth hormone receptors: regulation by retinoic acid.
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胚胎干细胞表达生长激素受体：受视黄酸调节。
DOI：
10.1210/endo.133.6.8243317
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发表时间：
1993
期刊：
影响因子：
4.8
通讯作者：
J. Törnell
中科院分区：
文献类型：
--
作者：
C. Ohlsson;K. Lövstedt;P. Holmes;A. Nilsson;L. Carlsson;J. Törnell
胚胎和胎儿生长通常被认为是独立于垂体生长激素。然而，最近有研究表明，18日龄的大鼠胚胎和大鼠胎儿表达生长激素受体，这表明生长激素可能在早期发育中发挥作用。本研究的目的是确定着床前胚胎是否也表达生长激素受体。采用Northern blot法、rnase保护液杂交法、逆转录/聚合酶链反应法和免疫组化法检测生长激素受体。使用与生长激素受体细胞外结构域对应的探针对胚胎干细胞进行Northern blot分析，发现存在两种转录本（1.2和4.5千碱基）。rnase保护溶液杂交实验显示，胚胎干细胞表达的gh受体信使RNA （mRNA）水平约为妊娠小鼠肝脏表达水平的六分之一。维甲酸（100 nM）处理胚胎干细胞6 d后，gh受体mRNA水平升高（P < 0.01）。通过逆转录/聚合酶链反应实验，进一步在胚胎干细胞、着床前胚胎、肌肉、肝脏和胎盘中鉴定gh受体mRNA。在人类中，先前已经证明gh受体的外显子3在胎盘中被删除。然而，所研究的小鼠组织中没有缺失与人类生长激素受体外显子3相对应的GH受体mRNA。采用免疫组化方法对培养的es细胞进行gh受体免疫反应性鉴定。总之，我们在本研究中表明，种系胚胎干细胞和胚胎植入前小鼠胚胎表达生长激素受体转录物，并且这种转录物在胚胎干细胞中被视黄酸增加。此外，gh受体免疫反应性在ES细胞上的存在表明gh受体转录物被翻译。
Embryonic and fetal growth are generally considered to be independent of pituitary GH. However, it has been demonstrated recently that 18-day-old rat embryos and rat fetuses express GH receptors, suggesting that GH could play a role in early development. The aim of the present investigation was to determine whether preimplantation embryos also express GH receptors. Germ line competent mouse embryonic stem (ES) cells and cultured mouse preimplantation embryos were examined with Northern blot analysis, RNAse-protection solution-hybridization assays, reverse transcription/polymerase chain reaction assays and immunohistochemistry for the detection of GH receptors. Northern blot analysis of ES cells using a probe corresponding to the extracellular domain of the GH receptor demonstrated the presence of two transcripts (1.2 and 4.5 kilobases). The RNAse-protection solution-hybridization assay revealed that ES cells express approximately one sixth of the GH-receptor messenger RNA (mRNA) levels expressed in liver from pregnant mice. Treatment of cultured ES cells with retinoic acid (100 nM) for 6 days increased GH-receptor mRNA levels (P < 0.01). GH-receptor mRNA was further identified in ES cells, preimplantation embryos, muscle, liver, and placenta by a reverse transcription/polymerase chain reaction assay. In humans it has previously been shown that exon 3 of the GH-receptor is deleted in the placenta. However, none of the studied mouse tissues had a deletion of the GH-receptor mRNA corresponding to exon 3 of the human GH receptor. GH-receptor immunoreactivity was identified on the cultured ES-cells by immunohistochemistry. In conclusion, we have in the present study shown that germline competent ES cells and preimplantation mouse embryos express the GH receptor transcript and that this transcription is increased by retinoic acid in ES cells. Furthermore, the presence of GH-receptor immunoreactivity on the ES cells indicates that the GH-receptor transcript is translated.
生长激素信使核糖核酸的反义寡脱氧核苷酸抑制淋巴细胞增殖。
影响因子：
4.8
作者：
Weigent,DA;Blalock,JE;LeBoeuf,RD
通讯作者：
LeBoeuf,RD
DOI：
10.1073/pnas.86.20.8083
发表时间：
1989-10-01
影响因子：
11.1
作者：
GODOWSKI, PJ;LEUNG, DW;WOOD, WI
通讯作者：
WOOD, WI
DOI：
10.1073/pnas.83.24.9343
发表时间：
1986-12-01
影响因子：
11.1
作者：
MATHEWS, LS;NORSTEDT, G;PALMITER, RD
通讯作者：
PALMITER, RD