Expression changes of duplicated genes in allotetraploids of Brassica detected by SRAP-cDNA technique

Expression changes of duplicated genes in allotetraploids of Brassica detected by SRAP-cDNA technique
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SRAP-cDNA技术检测甘蓝异源四倍体重复基因的表达变化

DOI:
10.1134/s0026893309010014
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发表时间:
2009-02
期刊:
影响因子:
1.2
通讯作者:
--
中科院分区:
生物学4区
文献类型:
--
作者:

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多倍体化在植物进化过程中起着重要的作用,它可能引发基因组结构和基因表达的变化。本研究的目的是检测天然芸苔异源四倍体基因表达改变的程度。对6个种属(13份)、3个异源四倍体及其3个祖先二倍体进行了序列相关扩增多态性(SRAP)-cDNA显示。共筛选1496个转录本,发现92个(6.1%)转录本在异源多倍体中被沉默,19个(1.3%)转录本被激活。
Polyploidization plays an important role in plant evolution, and it may trigger changes in genome structure and gene expression. The purpose of this study was to detect the extent of gene expression alterations in natural Brassica allotetraploids. Sequence-related amplified polymorphism (SRAP)-cDNA display was carried out on six species (13 accessions), three allotetraploids and their three ancestral diploids. Total of 1496 transcripts were screened, finding that 92 (6.1%) transcripts were subjected to silencing in allopolyploids, while 19 (1.3%) transcripts were activated.
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