The sigma factor σ(54) is required for the long-term survival of Leptospira biflexa in water.
The sigma factor σ(54) is required for the long-term survival of Leptospira biflexa in water.
复制标题
双曲钩端螺旋体在水中长期生存需要 sigma 因子 sigma(54)。
DOI：
10.1111/mmi.13967
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发表时间：
2018-04-06
影响因子：
3.6
通讯作者：
Yang XF
中科院分区：
文献类型：
--
作者：
Zhang JJ;Hu WL;Yang Y;Li H;Picardeau M;Yan J;Yang XF
钩端螺旋体包括致病的和自由生活的寄生物种。钩端螺旋体的环境适应和生存机制知之甚少。已知替代σ因子σ54（RpoN）在许多细菌的环境和宿主适应中起重要作用。本研究通过等位基因交换的方法构建了rpoN突变株，并将其在红曲霉中进行了互补。biflexa。转录组分析表明，参与氮吸收和代谢的几个基因的表达，包括amtB 1，glnB-amtB 2，ntrX和narK，由σ54控制。野生型L. biflexa不能在氮限制条件下生长，但能够在这种条件下存活并迅速恢复，而rpoN突变体不能。rpoN突变体在水中长期生存的能力也大大降低。σ54似乎以间接方式调节amtB 1、glnK-amtB 2、ntrX和narK的表达。然而，我们鉴定了一个新的氮相关基因LEPBI_I1011，其表达直接受σ54（在此将RpoN控制的因子A重命名为rcfA）的控制。综上所述，我们的数据表明，σ54调节网络在钩端螺旋体的长期环境生存中起着重要作用。钩端螺旋体的环境适应和生存机制知之甚少。本研究报告了双曲钩端螺旋体中编码替代σ因子σ54的rpoN的失活和互补，并证明σ54是钩端螺旋体在水中长期存活所必需的。
Leptospira spp. comprise both pathogenic and free-living saprophytic species. Little is known about the environmental adaptation and survival mechanisms of Leptospira. Alternative sigma factor, σ54 (RpoN) is known to play an important role in environmental and host adaptation in many bacteria. In this study, we constructed an rpoN mutant by allele exchange, and the complemented strain in saprophytic L. biflexa. Transcriptome analysis revealed that expression of several genes involved in nitrogen uptake and metabolism, including amtB1, glnB-amtB2, ntrX, and narK, were controlled by σ54. While wild-type L. biflexa could not grow under nitrogen-limiting conditions but was able to survive under such conditions and recover rapidly, the rpoN mutant was not. The rpoN mutant also had dramatically reduced ability to survive long-term in water. σ54 appears to regulate expression of amtB1, glnK-amtB2, ntrX, and narK in an indirect manner. However, we identified a novel nitrogen-related gene, LEPBI_I1011, whose expression was directly under the control of σ54 (herein renamed as rcfA for RpoN-controlled factor A). Taken together, our data reveal that the σ54 regulatory network plays an important role in the long-term environmental survival of Leptospira spp. Little is known about the environmental adaptation and survival mechanisms of Leptospira. This study reports inactivation and complementation of rpoN encoding the alternative sigma factor σ54 in Leptospira biflexa, and demonstrates that σ54 is required for the long term survival in water of leptospires.
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DOI：
10.1093/bioinformatics/8.3.275
发表时间：
1992-06-01
期刊：
COMPUTER APPLICATIONS IN THE BIOSCIENCES
影响因子：
--
作者：
JONES, DT;TAYLOR, WR;THORNTON, JM
通讯作者：
THORNTON, JM
影响因子：
6.7
作者：
Caimano MJ;Sivasankaran SK;Allard A;Hurley D;Hokamp K;Grassmann AA;Hinton JC;Nally JE
通讯作者：
Nally JE
影响因子：
3.2
作者：
HOPPER, S;BOCK, A
通讯作者：
BOCK, A
影响因子：
4.8
作者：
Javelle, A;Severi, E;Merrick, M
通讯作者：
Merrick, M
影响因子：
3.2
作者：
Cuñé, J;Cullen, P;Barbe, J
通讯作者：
Barbe, J