Lipidomics in Understanding Pathophysiology and Pharmacologic Effects in Inflammatory Diseases: Considerations for Drug Development.
Lipidomics in Understanding Pathophysiology and Pharmacologic Effects in Inflammatory Diseases: Considerations for Drug Development.
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DOI：
10.3390/metabo12040333
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发表时间：
2022-04-07
期刊：
影响因子：
4.1
通讯作者：
Asante, Isaac
中科院分区：
文献类型：
--
作者：
Ahluwalia, Kabir;Ebright, Brandon;Chow, Kingsley;Dave, Priyal;Mead, Andrew;Poblete, Roy;Louie, Stan G.;Asante, Isaac
关键词：
脂质体具有广泛的生物学和信号功能，包括作为膜的结构支架以及启动和化解炎症。为了研究磷脂及其生物活性代谢物的生物活性，需要精确的分析技术来鉴定特定的脂类并量化它们的水平。利用高灵敏质谱仪(MS)技术可以实现一组脂质的同时定量，该技术的技术进步在过去十年中有了显著的进步。这释放了代谢组学/脂质组学的力量，允许对代谢系统进行动态表征。脂质组学是代谢组学的一个子集，用于内源性脂质及其代谢物的多分析鉴定和定量。基于脂质组学的技术具有推动新的生物标记物发现和治疗开发计划的潜力；然而，该领域尚未建立适当的标准。标准化将改进脂组分析，并加速创新疗法的开发。本综述旨在总结脂肪组学研究设计的考虑因素，包括仪器、样本稳定性、数据验证和数据分析。此外，本文还重点介绍了脂质组学如何应用于心血管疾病、神经退行性疾病、肺部疾病和自身免疫性疾病等各种炎症性疾病的生物标记物发现和药物机制剖析。
The lipidome has a broad range of biological and signaling functions, including serving as a structural scaffold for membranes and initiating and resolving inflammation. To investigate the biological activity of phospholipids and their bioactive metabolites, precise analytical techniques are necessary to identify specific lipids and quantify their levels. Simultaneous quantification of a set of lipids can be achieved using high sensitivity mass spectrometry (MS) techniques, whose technological advancements have significantly improved over the last decade. This has unlocked the power of metabolomics/lipidomics allowing the dynamic characterization of metabolic systems. Lipidomics is a subset of metabolomics for multianalyte identification and quantification of endogenous lipids and their metabolites. Lipidomics-based technology has the potential to drive novel biomarker discovery and therapeutic development programs; however, appropriate standards have not been established for the field. Standardization would improve lipidomic analyses and accelerate the development of innovative therapies. This review aims to summarize considerations for lipidomic study designs including instrumentation, sample stabilization, data validation, and data analysis. In addition, this review highlights how lipidomics can be applied to biomarker discovery and drug mechanism dissection in various inflammatory diseases including cardiovascular disease, neurodegeneration, lung disease, and autoimmune disease.
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影响因子：
3.6
作者：
Bowden, John A.;Ulmer, Candice Z.;Yost, Richard A.
通讯作者：
Yost, Richard A.
影响因子：
12.4
作者：
Biemmi, Vanessa;Milano, Giuseppina;Barile, Lucio
通讯作者：
Barile, Lucio
DOI：
10.1096/fj.202100540r
发表时间：
2021-06
期刊：
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
影响因子：
--
作者：
Archambault AS;Zaid Y;Rakotoarivelo V;Turcotte C;Doré É;Dubuc I;Martin C;Flamand O;Amar Y;Cheikh A;Fares H;El Hassani A;Tijani Y;Côté A;Laviolette M;Boilard É;Flamand L;Flamand N
通讯作者：
Flamand N
影响因子：
6.7
作者：
Bitto, Alessandra;Giuliani, Daniela;Altavilla, Domenica
通讯作者：
Altavilla, Domenica
影响因子：
2.3
作者：
Bergheanu, Sandrin C.;Reijmers, Theo;Jukema, J. Wouter
通讯作者：
Jukema, J. Wouter