Identification of a Fatty Acid for Diagnosing Non-Alcoholic Steatohepatitis in Patients with Severe Obesity Undergoing Metabolic Surgery.
Identification of a Fatty Acid for Diagnosing Non-Alcoholic Steatohepatitis in Patients with Severe Obesity Undergoing Metabolic Surgery.
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DOI：
10.3390/biomedicines10112920
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发表时间：
2022-11-14
期刊：
影响因子：
4.7
通讯作者：
中科院分区：
文献类型：
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在严重肥胖的日本患者中，非酒精性脂肪性肝炎（NASH）的患病率极高。然而，目前除了肝活检外，没有其他方法来评估肝脂肪变性和纤维化。本研究的目的是全面分析严重肥胖日本患者的脂肪酸（FA）和血清游离脂肪酸（FFA）代谢的变化，以确定这些是否可以作为替代标志物。在这项研究中，我们招募了20名日本患者，他们接受了腹腔镜袖状胃切除术（LSG）治疗重度肥胖和术中肝活检。用液相色谱-质谱法分析血清FFA，并使用基质辅助激光解吸/电离成像质谱法评估肝组织中的FA，以确定可能指示阳性NASH诊断的FA。所有患者在LSG后均表现出显著的体重减轻和代谢改善。关于体重减轻和代谢改善指标，23个FFA显示与基线数据显著相关。将磷脂缩小到肝组织中检测到的常见FA，PC（18：1e_20：4）在NASH组中显著改变，表明其可用作NASH诊断的替代标志物。结果表明，血液磷脂的特定术后变化可用作NASH治疗的替代标志物。
The prevalence of nonalcoholic steatohepatitis (NASH) in severely obese Japanese patients is extremely high. However, there are currently no methods other than liver biopsy to assess hepatic steatosis and fibrosis. The purpose of this study was to comprehensively analyze changes in fatty acid (FA) and serum-free fatty acid (FFA) metabolism in severely obese Japanese patients to determine whether these could be surrogate markers. In this study, we enrolled 20 Japanese patients who underwent laparoscopic sleeve gastrectomy (LSG) for severe obesity and intraoperative liver biopsy. Serum FFAs were analyzed with liquid chromatography-mass spectrometry, and FAs in liver tissue were assessed using matrix-assisted laser desorption/ionization-imaging mass spectrometry to determine FAs that may be indicative of a positive NASH diagnosis. All patients showed significant weight loss and metabolic improvement following LSG. Regarding weight loss and metabolic improvement indices, 23 FFAs showed significant correlations with the baseline data. Narrowing down the phospholipids to commonly detected FAs detected in liver tissue, PC(18:1e_20:4) was significantly changed in the NASH group, suggesting that it could be used as a surrogate marker for NASH diagnosis. The results suggest that specific postoperative changes in blood phospholipids could be used as surrogate markers for NASH treatment.
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影响因子：
120.7
作者：
Flegal, Katherine M.;Carroll, Margaret D.;Curtin, Lester R.
通讯作者：
Curtin, Lester R.
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