Prevalence of basal core promoter and precore mutations in Chinese chronic hepatitis B patients and correlation with serum HBeAG titers.
Prevalence of basal core promoter and precore mutations in Chinese chronic hepatitis B patients and correlation with serum HBeAG titers.
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DOI：
10.1002/jmv.21439
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发表时间：
2009-05
影响因子：
12.7
通讯作者：
Tong S
中科院分区：
文献类型：
--
作者：
Qin Y;Zhang J;Mao R;Guo H;Yin Y;Wu X;Weng X;Wands J;Tong S
乙肝病毒基因组基本核心启动子(BCP)区的A1762T和G1764A突变以及前C区的G1896A突变在HBeAg阴性患者中较为常见。体外实验表明，BCP和PC突变分别降低和取消了HBeAg的表达。对中国地区2 0 7例HBeAg阳性慢性乙型肝炎患者进行了BCP和PC基因突变检测，并与血清HBeAg滴度进行了相关分析。所有患者均未接受抗病毒治疗。乙肝病毒的基因分型采用直接测序的方法。用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)检测BCP和PC突变，并经DNA测序证实。用微粒子酶免疫法测定HBeAg滴度。207例患者中，B型51例(24.6%)，C型1例，BCP突变103例(50%)，PC突变43例(20.8%)。13例患者(6.3%)同时存在BCP和PC突变。感染这两种基因的患者之间HBeAg滴度无显著差异，但BCP或PC突变的存在与HBeAg滴度降低有关(P<；0.05)。同时存在BCP和PC突变时，HBeAg滴度甚至更低(P<；0.05)。这些发现证实，在HBeAg患者中，BCP和PC突变降低了HBeAg的表达。
The A1762T and G1764A mutations in the basal core promoter (BCP) region and the G1896A mutation in the precore (PC) region of hepatitis B virus (HBV) genome are found commonly in HBeAg-negative patients. Experiments in vitro suggest that BCP and PC mutation reduce and abolish HBeAg expression, respectively. In the present study, the prevalence of the BCP and PC mutations were determined in 207 patients with HBeAg positive chronic hepatitis B from China and correlated with the titers of serum HBeAg. None of the patients received antiviral therapy. The HBV genotype was determined by direct sequencing of the HBsAg gene. The BCP and PC mutations were detected by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and confirmed by DNA sequencing. The HBeAg titer was measured by the microparticle enzyme immunoassay. Fifty-one of the 207 patients (24.6%) were infected with genotype B and the remainder with genotype C. The BCP mutations were detected in 103 patients (50%) while the PC mutation was present in 43 (20.8%). Thirteen patients (6.3%) harbored both BCP and PC mutations. No significant difference in the titers of HBeAg was found between patients infected with the two HBV genotypes, but the presence of either the BCP or PC mutation was associated with reduced HBeAg titer (P < 0.05). The presence of both the BCP and PC mutations was accompanied by even lower HBeAg titer (P < 0.05). These findings confirm that in patients with HBeAg, the BCP and PC mutations reduced the expression of HBeAg.
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影响因子：
8.8
作者：
Kirschberg, O;Schüttler, C;Schaefer, S
通讯作者：
Schaefer, S
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Chu, CJ;Keeffe, EB;Lok, ASF
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