Platelet-rich fibrin constructs elute higher concentrations of transforming growth factor-β1 and increase tendon cell proliferation over time when compared to blood clots: a comparative in vitro analysis.
Platelet-rich fibrin constructs elute higher concentrations of transforming growth factor-β1 and increase tendon cell proliferation over time when compared to blood clots: a comparative in vitro analysis.
复制标题
与血凝块相比，富含血小板的纤维蛋白结构可以洗脱更高浓度的转化生长因子-β1，并随着时间的推移增加肌腱细胞的增殖：体外比较分析。
作者：
L. Visser;S. Arnoczky;Oscar Caballero;M. Egerbacher
客观的
OBJECTIVE
To compare the concentration of a representative growth factor (transforming growth factor-beta [TGF-β]1) eluted from a platelet-rich fibrin matrix (PRFMatrix), a platelet-rich fibrin membrane (PRFMembrane), and a whole blood clot (BC) over time, and to compare the mitogenic effect of the eluents from each construct.
STUDY DESIGN
In vitro study.
SAMPLE POPULATION
PRFMatrix, PRFMembrane, and BC (n=4/construct/time point).
METHODS
Each construct was placed in tissue culture wells containing media for 7 days. The media was collected and replenished on days 1, 3, 5, and 7 and the concentration of eluted TGF-β1 was measured by enzyme-linked immunosorbent assay. Canine tendon cells were subjected to additional aliquots of the conditioned media and the amount of cell proliferation compared.
RESULTS
The media from both PRFM (PRFMatrix and PRFMembrane) constructs contained significantly more (P≤.026) TGF-β1 at days 1 and 3 and produced a significant increase (P≤.044) in cell proliferation at all time points compared with the BC. The PRFMembrane media contained significantly more (P≤.05) TGF-β1 at days 1 and 3 and produced a significant increase (P≤.002) in cell proliferation at all time points compared with the PRFMatrix.
CONCLUSIONS
Both PRFM constructs are comprised of a dense fibrin scaffold that contains increased concentrations of TGF-β1 and are capable of increasing tendon cell proliferation over time when compared with a BC.
CLINICAL RELEVANCE
The sustained increase in growth factor availability in PRFM constructs may be beneficial in the healing of biologically compromised tissues.
影响因子：
1.9
作者：
Thomopoulos, S;Harwood, FL;Gelberman, RH
通讯作者：
Gelberman, RH