Cloning and characterization of Escherichia coli DUF299: a bifunctional ADP-dependent kinase--Pi-dependent pyrophosphorylase from bacteria.
Cloning and characterization of Escherichia coli DUF299: a bifunctional ADP-dependent kinase--Pi-dependent pyrophosphorylase from bacteria.
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DOI：
10.1186/1471-2091-11-1
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发表时间：
2010-01-03
期刊：
影响因子：
--
通讯作者：
Burnell JN
中科院分区：
文献类型：
--
作者：
Burnell JN
磷酸烯醇丙酮酸合成酶（PEPS; EC 2.7.9.2）在大肠杆菌中催化丙酮酸由磷酸烯醇丙酮酸合成磷酸烯醇丙酮酸。它还在糖异生中催化丙酮酸向磷酸烯醇丙酮酸的合成代谢转化。在成功克隆和表达玉米叶片丙酮酸正磷酸二激酶调控蛋白（PDRP）后，进行了生物信息学搜索，发现在300多种细菌中存在PDRP同源物；PDRP同源物鉴定为DUF299。本文报道了大肠杆菌中PEPS和DUF299的克隆和表达，证实了大肠杆菌DUF299既能催化PEPS的adp依赖性失活，也能催化PEPS的pi依赖性活化。本文首次报道了一种双功能调节酶在细菌中催化adp依赖性磷酸化和pi依赖性焦磷酸化反应。
Phosphoenolpyruvate synthetase (PEPS; EC 2.7.9.2) catalyzes the synthesis of phosphoenolpyruvate from pyruvate in Escherichia coli when cells are grown on a three carbon source. It also catalyses the anabolic conversion of pyruvate to phosphoenolpyruvate in gluconeogenesis. A bioinformatics search conducted following the successful cloning and expression of maize leaf pyruvate, orthophosphate dikinase regulatory protein (PDRP) revealed the presence of PDRP homologs in more than 300 bacterial species; the PDRP homolog was identified as DUF299. This paper describes the cloning and expression of both PEPS and DUF299 from E. coli and establishes that E. coli DUF299 catalyzes both the ADP-dependent inactivation and the Pi-dependent activation of PEPS. This paper represents the first report of a bifunctional regulatory enzyme catalysing an ADP-dependent phosphorylation and a Pi-dependent pyrophosphorylation reaction in bacteria.
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影响因子：
2.9
作者：
Chandran, SS;Yi, J;Frost, JW
通讯作者：
Frost, JW
影响因子：
7.2
作者：
Chastain, Chris J.;Xu, Wenxin;Chollet, Raymond
通讯作者：
Chollet, Raymond
影响因子：
3.6
作者：
Tjaden, B;Plagens, A;Hensel, R
通讯作者：
Hensel, R
DOI：
10.1073/pnas.79.19.5828
发表时间：
1982-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
OGINO, T;GARNER, C;HERRMANN, KM
通讯作者：
HERRMANN, KM
影响因子：
64.8
作者：
LAEMMLI, UK
通讯作者：
LAEMMLI, UK