Nonsense-mediated decay serves as a general viral restriction mechanism in plants.
Nonsense-mediated decay serves as a general viral restriction mechanism in plants.
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DOI：
10.1016/j.chom.2014.08.001
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发表时间：
2014-09-10
影响因子：
30.3
通讯作者：
Voinnet O
中科院分区：
文献类型：
--
作者：
Garcia D;Garcia S;Voinnet O
（+）链RNA病毒必须克服宿主中的各种限制点以建立成功的感染。在植物中，这包括RNA沉默。为了揭示RNA病毒感染的其他瓶颈，我们在遗传上减弱了RNA沉默对转基因表达的马铃薯X病毒（PVX）的影响，PVX是一种在拟南芥中复制的（+）链RNA病毒。在这种致敏背景下的遗传筛选揭示了无义介导的衰变（NMD），宿主RNA质量控制机制，如何识别和消除具有内部终止密码子和长3′ UTR的PVX RNA。NMD也在自然感染环境中起作用，虽然一些病毒已经进化出基因组表达策略来完全克服这一过程，但NMD激活病毒的毒力需要它们直接抑制NMD或促进NMD不利的细胞状态的能力。这些诱导、逃避和抑制的原理将NMD定义为植物中的一般病毒限制机制，其也可能在动物中起作用。拟南芥中（+）链RNA病毒积累修饰因子的致敏遗传筛选宿主无义介导的衰变（NMD）途径在自然感染期间限制PVX NMD靶向含有内部终止密码子和长3′ UTR的病毒RNA。确定无义介导的衰变（NMD）途径，内源RNA质量控制机制，作为RNA病毒感染植物的一般瓶颈。他们阐明了RNA病毒生物学的内在特征，这些特征在真实感染期间触发NMD，并表明病毒已经进化到逃避或抑制这一过程，以便在宿主中茁壮成长。
(+)strand RNA viruses have to overcome various points of restriction in the host to establish successful infection. In plants, this includes RNA silencing. To uncover additional bottlenecks to RNA virus infection, we genetically attenuated the impact of RNA silencing on transgenically expressed Potato virus X (PVX), a (+)strand RNA virus that replicates in Arabidopsis. A genetic screen in this sensitized background uncovered how nonsense-mediated decay (NMD), a host RNA quality control mechanism, recognizes and eliminates PVX RNAs with internal termination codons and long 3′ UTRs. NMD also operates in natural infection contexts, and while some viruses have evolved genome expression strategies to overcome this process altogether, the virulence of NMD-activating viruses entails their ability to directly suppress NMD or to promote an NMD-unfavorable cellular state. These principles of induction, evasion, and suppression define NMD as a general viral restriction mechanism in plants that also likely operates in animals. A sensitized genetic screen for modifiers of (+)strand RNA virus accumulation in Arabidopsis The host nonsense-mediated decay (NMD) pathway restricts PVX during natural infection NMD targets viral RNAs containing internal termination codons and long 3′ UTRs Some viruses have evolved to evade NMD altogether, while others may suppress NMD actively Garcia et al. identify the nonsense-mediated decay (NMD) pathway, an endogenous RNA quality control mechanism, as a general bottleneck to RNA virus infection in plants. They elucidate intrinsic features of RNA virus biology that trigger NMD during authentic infection and show that viruses have evolved to evade or suppress this process in order to thrive in their hosts.
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DOI：
10.1038/nrm3454
发表时间：
2012-11
期刊：
Nature reviews. Molecular cell biology
影响因子：
--
作者：
通讯作者：
--
影响因子：
10.5
作者：
Azevedo, Jacinthe;Garcia, Damien;Voinnet, Olivier
通讯作者：
Voinnet, Olivier
影响因子：
11.4
作者：
Dalmay, T;Horsefield, R;Baulcombe, DC
通讯作者：
Baulcombe, DC
影响因子：
64.5
作者：
Isken, Olaf;Kim, Yoon Ki;Maquat, Lynne E.
通讯作者：
Maquat, Lynne E.
影响因子：
11.4
作者：
Le Hir, H;Gatfield, D;Moore, MJ
通讯作者：
Moore, MJ