Tall fescue endophyte effects on tolerance to water-deficit stress.
Tall fescue endophyte effects on tolerance to water-deficit stress.
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DOI：
10.1186/1471-2229-13-127
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发表时间：
2013-09-09
影响因子：
5.3
通讯作者：
Schardl CL
中科院分区：
文献类型：
--
作者：
Nagabhyru P;Dinkins RD;Wood CL;Bacon CW;Schardl CL
内生真菌Neotyphodium coenophialum可以增强其寄主草高羊茅的耐旱性。为了研究内生菌对植物对急性缺水胁迫反应的影响，我们对具有内生菌（E+）和无内生菌（E-）的基因相同的高羊茅克隆对的芽和根组织中植物代谢物水平进行了全面分析，以响应直接缺水胁迫。通过用杀真菌剂处理E+植物并选择性地繁殖单分蘖来产生E-克隆。在 E+ 和 E- 克隆的时间进程研究中，0 至 5 天禁水，在此期间测定游离糖、糖醇和氨基酸的水平，以及一些主要真菌代谢物的水平。停水 2-3 天后，E+ 克隆的再浇水植物的存活率和分蘖率显着高于 E- 克隆。在停水两到三天内，内生菌对代谢物的显着影响表现为芽和根中游离葡萄糖、果糖、海藻糖、糖醇、脯氨酸和谷氨酸含量较高。真菌代谢物甘露醇和黑麦草碱生物碱也随着缺水而显着增加。我们的结果表明，共生的 N. coenophialum 有助于高羊茅植物从缺水中生存和恢复，并在一定程度上通过在施加压力后不久诱导这些相容性溶质的快速积累来发挥作用。
The endophytic fungus, Neotyphodium coenophialum, can enhance drought tolerance of its host grass, tall fescue. To investigate endophyte effects on plant responses to acute water deficit stress, we did comprehensive profiling of plant metabolite levels in both shoot and root tissues of genetically identical clone pairs of tall fescue with endophyte (E+) and without endophyte (E-) in response to direct water deficit stress. The E- clones were generated by treating E+ plants with fungicide and selectively propagating single tillers. In time course studies on the E+ and E- clones, water was withheld from 0 to 5 days, during which levels of free sugars, sugar alcohols, and amino acids were determined, as were levels of some major fungal metabolites. After 2–3 days of withholding water, survival and tillering of re-watered plants was significantly greater for E+ than E- clones. Within two to three days of withholding water, significant endophyte effects on metabolites manifested as higher levels of free glucose, fructose, trehalose, sugar alcohols, proline and glutamic acid in shoots and roots. The fungal metabolites, mannitol and loline alkaloids, also significantly increased with water deficit. Our results suggest that symbiotic N. coenophialum aids in survival and recovery of tall fescue plants from water deficit, and acts in part by inducing rapid accumulation of these compatible solutes soon after imposition of stress.
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影响因子：
4.4
作者：
AN, ZQ;SIEGEL, MR;SCHARDL, CL
通讯作者：
SCHARDL, CL
影响因子：
5.2
作者：
Cortina, C;Culiánez-Macià, FA
通讯作者：
Culiánez-Macià, FA
影响因子：
3.2
作者：
Blankenship, JD;Houseknecht, JB;Schardl, CL
通讯作者：
Schardl, CL
DOI：
10.1080/00288233.2006.9513726
发表时间：
2006-12-01
影响因子：
1.8
作者：
Assuero, S. G.;Tognetti, J. A.;Posse, M. A.
通讯作者：
Posse, M. A.
影响因子：
6.6
作者：
BACON, CW
通讯作者：
BACON, CW