Downregulation of connective tissue growth factor inhibits the growth and invasion of gastric cancer cells and attenuates peritoneal dissemination.
Downregulation of connective tissue growth factor inhibits the growth and invasion of gastric cancer cells and attenuates peritoneal dissemination.
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结缔组织生长因子的下调抑制胃癌细胞的生长和侵袭并减弱腹膜播散
DOI：
10.1186/1476-4598-10-122
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发表时间：
2011-09-28
期刊：
影响因子：
37.3
通讯作者：
Xu HM
中科院分区：
文献类型：
--
作者：
Jiang CG;Lv L;Liu FR;Wang ZN;Liu FN;Li YS;Wang CY;Zhang HY;Sun Z;Xu HM
背景结缔组织生长因子（CTGF）与肿瘤的发生、发展密切相关.然而，CTGF在胃癌中的作用仍然在很大程度上unknown.ResultsIn这项研究中，我们发现，CTGF在胃癌组织中高表达与匹配的正常胃组织相比。CTGF表达与肿瘤组织学分级、淋巴结转移和腹膜转移有关（P < 0.05）。CTGF表达阳性者术后5年累计生存率为22.9%，显著低于CTGF表达阴性者的48.1%（P < 0.001）。我们证明，敲低CTGF表达显着抑制胃癌细胞的细胞生长和细胞周期蛋白D1的表达减少。CTGF表达下调还可显著降低胃癌细胞的迁移和侵袭能力，并降低基质金属蛋白酶（MMP）-2和MMP-9的表达。动物实验表明，裸鼠注射CTGF敲除稳定的细胞系的腹膜种植结节的数量较少，比对照cells lines.ConclusionsThese数据表明，CTGF在细胞生长和侵袭在人胃癌中起着重要的作用，它似乎是一个潜在的胃癌患者的预后标志物。
BackgroundConnective tissue growth factor (CTGF) has been shown to be implicated in tumor development and progression. However, the role of CTGF in gastric cancer remains largely unknown.ResultsIn this study, we showed that CTGF was highly expressed in gastric cancer tissues compared with matched normal gastric tissues. The CTGF expression in tumor tissue was associated with histologic grade, lymph node metastasis and peritoneal dissemination (P < 0.05). Patients with positive CTGF expression had significantly lower cumulative postoperative 5 year survival rate than those with negative CTGF expression (22.9% versus 48.1%, P < 0.001). We demonstrated that knockdown of CTGF expression significantly inhibited cell growth of gastric cancer cells and decreased cyclin D1expression. Moreover, knockdown of CTGF expression also markedly reduced the migration and invasion of gastric cancer cells and decreased the expression of matrix metalloproteinase (MMP)-2 and MMP-9. Animal studies revealed that nude mice injected with the CTGF knockdown stable cell lines featured a smaller number of peritoneal seeding nodules than the control cell lines.ConclusionsThese data suggest that CTGF plays an important role in cell growth and invasion in human gastric cancer and it appears to be a potential prognostic marker for patients with gastric cancer.
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影响因子：
7.3
作者：
Jones, JL;Shaw, JA;Walker, RA
通讯作者：
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