Transcriptional Analysis of the Guinea Pig Mucosal Immune Response to Intravaginal Infection with Herpes Simplex Virus Type 2.
Transcriptional Analysis of the Guinea Pig Mucosal Immune Response to Intravaginal Infection with Herpes Simplex Virus Type 2.
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DOI：
10.1016/j.virol.2018.03.019
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发表时间：
2018-05
期刊：
影响因子：
3.7
通讯作者：
Bourne N
中科院分区：
文献类型：
--
作者：
Veselenak RL;Milligan GN;Miller AL;Pyles RB;Bourne N
豚鼠生殖器疱疹感染与人类感染密切相关，但免疫表征工具有限。利用基于pcr的阴道拭子样本阵列分析，研究了豚鼠阴道上皮表面对HSV感染的免疫力。IFNγ是整个感染过程中最显著上调的基因之一，根据干扰素分析，超过40%的表达显著改变的基因与IFNγ有关。通过RTPCR和IFNγ报告细胞证实了生殖粘膜中IFNγ转录物和生物活性。基因本体论分析揭示了人类和小鼠共有的许多与生殖器免疫相关的生物过程的激活，表明豚鼠和其他已建立的模型对原发性生殖器HSV-2感染的局部免疫反应具有相似性。这种基于转录的阵列将有助于在潜伏期再激活期间解剖免疫，这是其他动物模型无法很好地概括的感染结果。
Genital herpes infection in guinea pigs closely models human infection but tools for immune characterization are limited. Immunity to HSV infection at the vaginal epithelial surface was characterized in guinea pigs using PCR-based array analysis of vaginal swab samples. IFNγ was one of the most significantly upregulated genes throughout the infection and over 40% of genes with significantly altered expression were linked to IFNγ based on INTERFEROME analysis. IFNγ transcripts and biologically active IFNγ at the genital mucosa were confirmed by RTPCR and IFNγ reporter cells. Gene ontology analysis revealed activation of many biological processes related to genital immunity shared by humans and mice demonstrating the similarities of the local immune response to primary genital HSV-2 infection in guinea pigs and other established models. This transcription-based array will be useful for dissection of immunity during reactivation from latency, an infection outcome that is not well recapitulated by other animal models.
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影响因子：
3.7
作者：
Milligan, GN;Bernstein, DI
通讯作者：
Bernstein, DI
影响因子：
168.9
作者：
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