Diagnostic approach for cancer cells in urine sediments by 5-aminolevulinic acid-based photodynamic detection in bladder cancer.
Diagnostic approach for cancer cells in urine sediments by 5-aminolevulinic acid-based photodynamic detection in bladder cancer.
复制标题
DOI：
10.1111/cas.12393
复制
发表时间：
2014-05
期刊：
影响因子：
5.7
通讯作者：
Fujimoto K
中科院分区：
文献类型：
--
作者：
Miyake M;Nakai Y;Anai S;Tatsumi Y;Kuwada M;Onishi S;Chihara Y;Tanaka N;Hirao Y;Fujimoto K
膀胱尿路上皮癌的诊断和经尿道切除术后定期使用膀胱镜检查，尿细胞学和尿生物标志物的组合进行随访。然而，膀胱镜检查可能会忽略像原位癌这样的扁平病变，并且尿液检查对低度恶性肿瘤的敏感性较差。目前迫切需要一种客观、简便的尿液诊断检测方法来治疗膀胱癌。在这项研究中，使用了三种不同的模式，5-氨基乙酰丙酸（ALA）为基础的光动力学诊断测试。我们开发了一种紧凑型台式设备，定量细胞悬浮液中的红色荧光，称为“细胞荧光分析单元”（CFAU）。将58例膀胱癌患者的尿液样本离心，然后用ALA处理尿沉淀物。ALA处理的沉积物进行了三个荧光检测试验，包括CFAU测定。常规细胞学、BTA、NMP 22、荧光细胞学、荧光分光光度法和CFAU法的总敏感性分别为48%、33%、40%、86%、86%和87%。三种不同的基于ALA的测定显示出高灵敏度和特异性。基于ALA的检测以比常规尿细胞学、BTA和NMP 22（8-20%灵敏度）更高的比率（68-80%灵敏度）检测低级别和低阶段膀胱尿路上皮细胞。我们的研究结果表明，基于ALA的荧光检测分析是有前途的工具，用于膀胱癌的管理。为了避免繁琐的检测步骤和样品稳定性差所带来的变异性，有必要开发一种快速、自动化的ALA光动力学检测装置。
Bladder urothelial carcinoma is diagnosed and followed up after transurethral resection using a combination of cystoscopy, urine cytology and urine biomarkers at regular intervals. However, cystoscopy can overlook flat lesions like carcinoma in situ, and the sensitivity of urinary tests is poor in low-grade tumors. There is an emergent need for an objective and easy urinary diagnostic test for the management of bladder cancer. In this study, three different modalities for 5-aminolevulinic acid (ALA)-based photodynamic diagnostic tests were used. We developed a compact-size, desktop-type device quantifying red fluorescence in cell suspensions, named “Cellular Fluorescence Analysis Unit” (CFAU). Urine samples from 58 patients with bladder cancer were centrifuged, and urine sediments were then treated with ALA. ALA-treated sediments were subjected to three fluorescence detection assays, including the CFAU assay. The overall sensitivities of conventional cytology, BTA, NMP22, fluorescence cytology, fluorescent spectrophotometric assay and CFAU assay were 48%, 33%, 40%, 86%, 86% and 87%, respectively. Three different ALA-based assays showed high sensitivity and specificity. The ALA-based assay detected low-grade and low-stage bladder urothelial cells at shigher rate (68–80% sensitivity) than conventional urine cytology, BTA and NMP22 (8–20% sensitivity). Our findings demonstrate that the ALA-based fluorescence detection assay is promising tool for the management of bladder cancer. Development of a rapid and automated device for ALA-based photodynamic assay is necessary to avoid the variability induced by troublesome steps and low stability of specimens.
登录
查看更多内容
影响因子：
4
作者：
Goodison, Steve;Rosser, Charles J.;Urquidi, Virginia
通讯作者：
Urquidi, Virginia
影响因子：
4.5
作者：
Tauber, S;Stepp, H;Stief, C
通讯作者：
Stief, C
影响因子：
7.5
作者：
Barclay GR;Tura O;Samuel K;Hadoke PW;Mills NL;Newby DE;Turner ML
通讯作者：
Turner ML
影响因子：
23.4
作者：
Burger, Maximilian;Grossman, H. Barton;Jocham, Dieter
通讯作者：
Jocham, Dieter
DOI：
10.1016/j.urolonc.2013.06.001
发表时间：
2014-04-01
影响因子：
2.7
作者：
Xylinas, Evanguelos;Kluth, Luis A.;Shariat, Shahrokh F.
通讯作者：
Shariat, Shahrokh F.