A deep insight into the sialotranscriptome of the mosquito, Psorophora albipes.
A deep insight into the sialotranscriptome of the mosquito, Psorophora albipes.
复制标题
DOI：
10.1186/1471-2164-14-875
复制
发表时间：
2013-12-13
期刊：
影响因子：
4.4
通讯作者：
Ribeiro JM
中科院分区：
文献类型：
--
作者：
Chagas AC;Calvo E;Rios-Velásquez CM;Pessoa FA;Medeiros JF;Ribeiro JM
牛皮癣蚊子只在美洲发现，与脑炎和西尼罗河热病毒以及其他虫媒病毒的传播有关。苔藓唾液腺是许多寄生虫分化和传播的最终途径。它们还分泌具有强大药理作用的分子，可调节宿主止血、炎症和免疫反应。在这里，我们采用新一代测序和蛋白质组学方法，首次调查了一种Psorophora属蚊子的唾液成分。我们还讨论了这种蚊子属的进化位置到蚊科家庭通过比较其分泌的唾液化合物到目前为止确定的其他蚊子唾液蛋白的身份。Illumina测序产生13，535，229个序列读段，其组装成3，247个重叠群。所有家族根据其计算机预测的功能/活性进行分类。这些序列的注释允许其产品分为83个唾液蛋白家族，其中20个（24.39%）被我们随后的蛋白质组分析证实。两个蛋白质家族从伊蚊和一个Ochlerotatus中被分离出来，而四个蛋白质家族被描述为新的，因为它们与任何其他已知的蚊子唾液序列不匹配。几个蛋白质家族被描述为独有的库蚊存在于Psorophora蚊子，而我们没有确定任何成员的蛋白质家族已经被称为独特的按蚊。与伊蚊同源蛋白的同源性最高（69.23%），其次为黄绿蚊（8.15%）、库蚊（6.52%）和按蚊（4.66%）。这是第一个sialome（来自希腊sialo =唾液）目录的唾液蛋白从Psorophora蚊子，这可能是有用的，更好地了解这种蚊子的生命周期和作用，其唾液分泌在虫媒病毒传播。
Psorophora mosquitoes are exclusively found in the Americas and have been associated with transmission of encephalitis and West Nile fever viruses, among other arboviruses. Mosquito salivary glands represent the final route of differentiation and transmission of many parasites. They also secrete molecules with powerful pharmacologic actions that modulate host hemostasis, inflammation, and immune response. Here, we employed next generation sequencing and proteome approaches to investigate for the first time the salivary composition of a mosquito member of the Psorophora genus. We additionally discuss the evolutionary position of this mosquito genus into the Culicidae family by comparing the identity of its secreted salivary compounds to other mosquito salivary proteins identified so far. Illumina sequencing resulted in 13,535,229 sequence reads, which were assembled into 3,247 contigs. All families were classified according to their in silico-predicted function/ activity. Annotation of these sequences allowed classification of their products into 83 salivary protein families, twenty (24.39%) of which were confirmed by our subsequent proteome analysis. Two protein families were deorphanized from Aedes and one from Ochlerotatus, while four protein families were described as novel to Psorophora genus because they had no match with any other known mosquito salivary sequence. Several protein families described as exclusive to Culicines were present in Psorophora mosquitoes, while we did not identify any member of the protein families already known as unique to Anophelines. Also, the Psorophora salivary proteins had better identity to homologs in Aedes (69.23%), followed by Ochlerotatus (8.15%), Culex (6.52%), and Anopheles (4.66%), respectively. This is the first sialome (from the Greek sialo = saliva) catalog of salivary proteins from a Psorophora mosquito, which may be useful for better understanding the lifecycle of this mosquito and the role of its salivary secretion in arboviral transmission.
登录
查看更多内容
影响因子：
2.9
作者：
Francischetti, IMB;Valenzuela, JG;Ribeiro, JMC
通讯作者：
Ribeiro, JMC
影响因子：
3.8
作者：
Johnson, JK;Li, J;Christensen, BM
通讯作者：
Christensen, BM
影响因子：
3.7
作者：
Karim S;Singh P;Ribeiro JM
通讯作者：
Ribeiro JM
影响因子：
3.9
作者：
Albert, S;Bhattacharya, D;Simúth, J
通讯作者：
Simúth, J
影响因子：
4.8
作者：
Calvo, Eric;Mizurini, Daniella M.;Francischetti, Ivo M. B.
通讯作者：
Francischetti, Ivo M. B.