A deep insight into the sialotranscriptome of the gulf coast tick, Amblyomma maculatum.
A deep insight into the sialotranscriptome of the gulf coast tick, Amblyomma maculatum.
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DOI：
10.1371/journal.pone.0028525
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Ribeiro JM
中科院分区：
文献类型：
--
作者：
Karim S;Singh P;Ribeiro JM
吸血节肢动物的唾液含有拮抗其宿主止血的化合物，包括血小板聚集、血管收缩和血液凝固;这些生物体的唾液也具有抗炎和免疫调节特性。也许是因为宿主对这些化合物产生了积极的免疫反应，这些化合物的多样性甚至在相关的吸血物种中也很大。由于这些特性，唾液有助于吸血，并有助于建立可以在吸血期间传播的病原体。我们通过焦磷酸测序从不同进食时间的成年雌性斑钝眼蜱蜱的唾液腺cDNA文库获得了1，626，969个读段。该数据的组装产生了72，441个大于149个核苷酸的序列，从中提取了15，914个编码序列。其中，5，353个与来自国家生物技术信息中心的非冗余数据库中的最佳匹配具有>75%的覆盖率，从而允许将4，850个序列存入GenBank。附加说明的数据集以超链接电子表格的形式提供。目前已知的分泌蛋白有133个家族，其中大部分功能未知。这组蛋白质数据构成了一个新的蛋白质挖掘平台，用于发现针对A.斑疹和它们携带的疾病。
Saliva of blood sucking arthropods contains compounds that antagonize their hosts' hemostasis, which include platelet aggregation, vasoconstriction and blood clotting; saliva of these organisms also has anti-inflammatory and immunomodullatory properties. Perhaps because hosts mount an active immune response against these compounds, the diversity of these compounds is large even among related blood sucking species. Because of these properties, saliva helps blood feeding as well as help the establishment of pathogens that can be transmitted during blood feeding. We have obtained 1,626,969 reads by pyrosequencing a salivary gland cDNA library from adult females Amblyomma maculatum ticks at different times of feeding. Assembly of this data produced 72,441 sequences larger than 149 nucleotides from which 15,914 coding sequences were extracted. Of these, 5,353 had >75% coverage to their best match in the non-redundant database from the National Center for Biotechnology information, allowing for the deposition of 4,850 sequences to GenBank. The annotated data sets are available as hyperlinked spreadsheets. Putative secreted proteins were classified in 133 families, most of which have no known function. This data set of proteins constitutes a mining platform for novel pharmacologically active proteins and for uncovering vaccine targets against A. maculatum and the diseases they carry.
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影响因子：
6.4
作者：
Cavassani, KA;Aliberti, JC;Ferreira, BR
通讯作者：
Ferreira, BR
影响因子：
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Bateman A
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Fikrig, E
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作者：
Batista, Isabel F. C.;Chudzinski-Tavassi, Ana M.;Junqueira-de-Azevedo, Inacio L. M.
通讯作者：
Junqueira-de-Azevedo, Inacio L. M.