Systematic comparison of ultraviolet photodissociation and electron transfer dissociation for peptide anion characterization.
Systematic comparison of ultraviolet photodissociation and electron transfer dissociation for peptide anion characterization.
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DOI：
10.1007/s13361-012-0424-9
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发表时间：
2012-10
影响因子：
3.2
通讯作者：
Brodbelt, Jennifer S.
中科院分区：
文献类型：
--
作者：
Shaw, Jared B.;Madsen, James A.;Xu, Hua;Brodbelt, Jennifer S.
关键词：
对193 nm紫外光解离(UVPD)和负电子转移解离(NETD)进行了比较，以确定它们在表征酸性蛋白质组方面的有效性，包括序列覆盖分布(跨肽骨架的产物离子信号的测量)、序列覆盖百分比、骨架切割偏好以及相对于前体电荷状态的碎片差异。对于低电荷态(n≤2)，UVPD比NETD产生更多的诊断信息，但对于高电荷态，两种方法具有可比性。UVPD通常产生更多的序列特定产物阵列(除了a-和x-型离子之外，还有b-、y-、c-、z-、y-、d-和w型离子)，而NETD通常产生更简单的a/x型离子集。利用高pH流动相结合通过修改的MassMatrix算法自动搜索数据库，进行了蛋白质消化的LC-MS/UVPD和LC-MS/NETD分析。UVPD在独立搜索中的表现通常优于NETD，这是因为它能够有效地对较低和较高电荷状态进行排序，并具有快速激活时间。然而，当与传统的阳性模式CID相结合时，两种方法都产生了互补信息，与单独使用CID相比，序列覆盖率和独特的多肽识别显著增加。对酸性多肽的NETD和UVPD MS/MS方法进行了综合比较。
Ultraviolet photodissociation at 193 nm (UVPD) and negative electron transfer dissociation (NETD) were compared to establish their utility for characterizing acidic proteomes with respect to sequence coverage distributions (a measure of product ion signals across the peptide backbone), sequence coverage percentages, backbone cleavage preferences, and fragmentation differences relative to precursor charge state. UVPD yielded significantly more diagnostic information compared to NETD for lower charge states (n ≤ 2), but both methods were comparable for higher charged species. While UVPD often generated a more heterogeneous array of sequence-specific products (b-, y-, c-, z-, Y-, d-, and w-type ions in addition to a- and x- type ions), NETD usually created simpler sets of a/x-type ions. LC-MS/UVPD and LC-MS/NETD analysis of protein digests utilizing high pH mobile phases coupled with automated database searching via modified versions of the MassMatrix algorithm was undertaken. UVPD generally outperformed NETD in stand-alone searches due to its ability to efficiently sequence both lower and higher charge states with rapid activation times. However, when combined with traditional positive mode CID, both methods yielded complementary information with significantly increased sequence coverage percentages and unique peptide identifications over that of just CID alone. A comprehensive comparison of NETD and UVPD MS/MS methods is undertaken for acidic peptides.
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影响因子：
4.4
作者：
Madsen JA;Boutz DR;Brodbelt JS
通讯作者：
Brodbelt JS
影响因子：
4.3
作者：
Kjeldsen, F;Silivra, OA;Zubarev, RA
通讯作者：
Zubarev, RA
影响因子：
7.4
作者：
Hofstadler, SA;Sannes-Lowery, KA;Griffey, RH
通讯作者：
Griffey, RH
影响因子：
7.4
作者：
Gunawardena, Harsha P.;Emory, Joshua F.;McLuckey, Scott A.
通讯作者：
McLuckey, Scott A.
DOI：
10.1016/j.jasms.2009.08.021
发表时间：
2009-12-01
影响因子：
3.2
作者：
Kim, Tae-Young;Reilly, James P.
通讯作者：
Reilly, James P.