The effects of ethanol consumption on vasculogenesis potential in nonhuman primates.
The effects of ethanol consumption on vasculogenesis potential in nonhuman primates.
复制标题
乙醇消耗对非人灵长类动物血管生成潜力的影响。
DOI：
10.1111/j.1530-0277.2007.00558.x
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
Soker,Shay
中科院分区：
文献类型：
--
作者：
Williams,JKoudy;Baptista,PedroM;Daunais,JamesB;Szeliga,KendallT;Friedman,DavidP;Soker,Shay
背景：血管发生对于损伤或病变血管的保存和修复至关重要。酒精是年轻人中最常滥用的药物，但其对血管生长和修复的影响尚不清楚。血管修复的基础是内皮祖细胞（EPC）的募集，以帮助形成新的血管网络（血管发生）。因此，本研究的目的是测量乙醇消耗对非人灵长类动物EPCs的产生、动员和血管生成潜力的影响。方法：4至5岁（年轻成年）雄性恒河猴食用猴饲料和水（对照，n= 7），或饲料和水+乙醇（酒精，2.45 g/d，n= 7）12个月。 采集外周血（PB）和骨髓（BM）样本进行细胞表面抗原（CD 45、CD 31、CD 44、CD 133、VEGF-R2-或KDR）的荧光激活细胞分选分析;并在Matrigel包被的平板上进行毛细血管形成分析。结果：与对照组相比，酒精组猴的PB和BM中非造血基质细胞（CD 45 −）和推定的间充质祖细胞（CD 45 −/CD 44+）数量更多（p<0.05）。 此外，与对照组相比，酒精组的BM和PB中有更多的EPC（CD 45 −/CD 133 +/KDR+）（p<0.05）。 然而，酒精猴的EPCs在基质胶包被的平板上形成毛细血管的可能性低于对照猴（p<0.05）。结论：猴中乙醇消耗显著增加EPCs的产生和动员，但降低其形成毛细血管的能力。 这种影响的病理生理后果尚不清楚，但可能代表乙醇诱导的BM慢性应激，导致EPC。
Background:Vasculogenesis is essential to the preservation and repair of damaged or diseased vessels. Alcohol is the most commonly abused drug among young adults, but its effects on vessel growth and repair are unknown. The basis of vascular repair is endothelial progenitor cell (EPC) recruitment to assist in the formation of new vascular network (vasculogenesis). Therefore, the objective of this study was to measure the effects of ethanol consumption on the production, mobilization and vasculogenesis potential EPCs in nonhuman primates.Methods:Four to five year‐old (young adult) male rhesus monkeys consumed monkey chow and water (Control,n= 7), or chow and water + ethanol (Alcohol, 2.45 g/d,n= 7) for 12 months. Peripheral blood (PB) and bone marrow (BM) samples were collected for fluorescence‐activated cell‐sorting analysis of cell surface antigens (CD45, CD31, CD44, CD133, VEGF‐R2 – or KDR); and for capillary formation on Matrigel‐coated plates.Results:There were greater numbers of nonhematopoeitic stromal cells (CD45−) and putative mesenchymal progenitor cells (CD45−/CD44+) in the PB and BM of Alcohol versus Control monkeys (p< 0.05). Additionally, there were greater numbers of EPCs (CD45−/CD133+/KDR+) in the BM and PB of Alcohol versus Control monkeys (p< 0.05). However, the EPCs of Alcohol monkeys were less likely to form capillaries on matrigel‐coated plates than Control monkeys (p< 0.05).Conclusions:Ethanol consumption in monkeys markedly increased the production and mobilization of EPCs, but decreased their ability to form capillaries. The pathophysiologic consequences of such effects are unclear, but may represent an ethanol‐induced chronic stress on the BM, resulting in EPC.
使用高选择性双青霉胺环状脑啡肽类似物对大鼠大脑中 δ 阿片受体进行放射自显影定位。
影响因子：
5
作者：
Gulya,K;Gehlert,DR;Wamsley,JK;Mosberg,HI;Hruby,VJ;Duckles,SP;Yamamura,HI
通讯作者：
Yamamura,HI
DOI：
--
发表时间：
1983-11
期刊：
The Journal of pharmacology and experimental therapeutics
影响因子：
--
作者：
K. Chang;E. Wei;A. Killian;J. Chang
通讯作者：
K. Chang;E. Wei;A. Killian;J. Chang
DOI：
10.1073/pnas.77.10.6239
发表时间：
1980-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
GOODMAN, RR;SNYDER, SH;YOUNG, WS
通讯作者：
YOUNG, WS