Retinoic acid-binding protein, rhombomeres and the neural crest.
Retinoic acid-binding protein, rhombomeres and the neural crest.
复制标题
视黄酸结合蛋白、菱形核和神经嵴。
作者：
Malcolm Maden;P. Hunt;U. Eriksson;Atsushi Kuroiwa;Robb Krumlauf;Dennis Summerbell
我们已经调查了细胞视黄酸结合蛋白（CRABP）在鸡胚神经系统发育中的时空分布，以回答两个具体的问题：神经嵴细胞含有CRABP和CRABP阳性神经母细胞在哪里和何时首次出现在神经管？关于神经嵴，我们比较了CRABP染色与HNK-1染色（迁移神经嵴的标志物），发现它们确实共同定位，但头嵴和躯干嵴的表现略有不同。在头部区域的组织，如额鼻肿块和鳃弓，HNK-1免疫反应是强烈的，在早期阶段，但它消失的CRABP免疫反应出现。因此，两种染色模式不重叠，而是互补的。在躯干中，HNK-1和CRABP同时染色相同的细胞群，例如通过体节前半部迁移的细胞群。在神经管中，CRABP阳性神经母细胞首先出现在菱形脑神经褶皱关闭后，然后出现一个特定的模式的免疫反应性在后脑的菱形。标记的细胞存在于未来的脊髓，后菱脑到菱形节6和菱形节4，从而产生一个单一的条纹图案。这种模式是动态的，并且随着前菱形开始标记而逐渐变化。这种初始模式与小鼠中某些同源盒基因的排列相似，这促使我们研究鸡Hox-2.9基因的表达。我们发现，在第15阶段，该基因的表达模式是密切相关的CRABP。并对维甲酸、CRABP与同源异型盒基因的关系进行了讨论。
We have investigated by immunocytochemistry the spatial and temporal distribution of cellular retinoic acid-binding protein (CRABP) in the developing nervous system of the chick embryo in order to answer two specific questions: do neural crest cells contain CRABP and where and when do CRABP-positive neuroblasts first arise in the neural tube? With regard to the neural crest, we have compared CRABP staining with HNK-1 staining (a marker of migrating neural crest) and found that they do indeed co-localise, but cephalic and trunk crest behave slightly differently. In the cephalic region in tissues such as the frontonasal mass and branchial arches, HNK-1 immunoreactivity is intense at early stages, but it disappears as CRABP immunoreactivity appears. Thus the two staining patterns do not overlap, but are complementary. In the trunk, HNK-1 and CRABP stain the same cell populations at the same time, such as those migrating through the anterior halves of the somites. In the neural tube, CRABP-positive neuroblasts first appear in the rhombencephalon just after the neural folds close and then a particular pattern of immunoreactivity appears within the rhombomeres of the hindbrain. Labelled cells are present in the future spinal cord, the posterior rhombencephalon up to rhombomere 6 and in rhombomere 4 thus producing a single stripe pattern. This pattern is dynamic and gradually changes as anterior rhombomeres begin to label. The similarity of this initial pattern to the arrangement of certain homeobox genes in the mouse stimulated us to examine the expression of the chicken Hox-2.9 gene. We show that at stage 15 the pattern of expression of this gene is closely related to that of CRABP. The relationship between retinoic acid, CRABP and homeobox genes is discussed.
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细胞视黄酸结合蛋白和视黄酸在鸡胚发育中的作用。
影响因子：
2.7
作者：
Maden,M;Ong,DE;Summerbell,D;Chytil,F;Hirst,EA
通讯作者：
Hirst,EA
脊椎动物肢体形态发生的分子方法。
DOI：
10.1242/dev.107.supplement.121
发表时间：
1989
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Smith,SM;Pang,K;Sundin,O;Wedden,SE;Thaller,C;Eichele,G
通讯作者：
Eichele,G
类视黄醇结合蛋白在发育中的哺乳动物神经系统中的分布。
DOI：
10.1242/dev.109.1.75
发表时间：
1990
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Maden,M;Ong,DE;Chytil,F
通讯作者：
Chytil,F
大鼠组织细胞质中细胞视黄醇和细胞视黄酸结合蛋白的放射免疫化学测定。
DOI：
--
发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Ong,DE;Crow,JA;Chytil,F
通讯作者：
Chytil,F