Hg2+ and Ni2+ alter induction of heat shock protein-72 in THP-1 human monocytes.
Hg2+ and Ni2+ alter induction of heat shock protein-72 in THP-1 human monocytes.
复制标题
Hg2 和 Ni2 改变 THP-1 人单核细胞中热休克蛋白 72 的诱导。
DOI：
10.1002/jbm.a.10100
复制
发表时间：
2003
期刊：
影响因子：
--
通讯作者：
H. Sano
中科院分区：
文献类型：
--
作者：
M. Noda;J. Wataha;J. B. Lewis;P. Lockwood;H. Komatsu;H. Sano
从生物医学合金中释放出的金属离子，特别是Ni（2+）和Hg（2+），所导致的生物负债仍然是一个令人担忧的问题。热休克蛋白（HSP）是一类在细胞应激（包括氧化应激）条件下可被诱导的分子伴侣。我们的假设是，由于Hg（2+）和Ni（2+）改变其他细胞应激反应，如谷胱甘肽水平和细胞因子分泌，这些金属离子可能会改变单核细胞中的HSP诱导，这是组织对生物医学合金反应的关键细胞。将THP-1单核细胞暴露于亚致死浓度的Hg（2+）或Ni（2+）1小时，伴或不伴热应激（43 ℃），然后在37 ℃下恢复2-6小时。HSP 72用免疫印迹法和荧光定量法测定。2-10 μ mol/L Hg（2+）暴露诱导HSP 72的表达，而无热应激。与热应激相比，HSP 72水平被Hg（2+）改变。该响应依赖于Hg（2+）的浓度和恢复时间。10 μ mol/L的Hg（2+）使HSP 72水平均匀降低。20-100 μ mol/L的Ni（2+）暴露在没有热应激的情况下不能诱导HSP 72的表达，但显著改变了热诱导的HSP 72表达，在40和100 μ mol/L的单独热处理下表达显著增加。从目前的研究结果支持的假设，这些金属离子可以在相关的浓度释放的生物医学合金，调节HSP在人单核细胞的表达。HSP表达的调节表明细胞应激的早期迹象，这可能对含有和释放这些金属离子的生物医学合金的整体生物反应是重要的。
The biological liabilities that result from the release of metal ions from biomedical alloys, particularly Ni(2+) and Hg(2+), continue to be a concern. Heat-shock proteins (HSP) are a class of molecular chaperones that may be induced under conditions of cellular stress, including oxidative stress. Our hypothesis was that because Hg(2+) and Ni(2+) alter other cellular stress responses such as glutathione levels and cytokine secretion, these metal ions may alter HSP induction in monocytes, which are key cells in the response of tissues to biomedical alloys. THP-1 monocytes were exposed to sublethal concentrations of Hg(2+) or Ni(2+) for 1 h with or without heat stress (43 degrees C), then allowed to recover at 37 degrees C for 2-6 h. HSP72 was measured using immunoblotting with phosphorimage quantification. Hg(2+) exposures of 2-10 micromol/L induced HSP72 without heat stress. With heat stress, HSP72 levels were altered by Hg(2+) versus heat stress alone. The response depended on the concentration of Hg(2+) and the recovery time. Hg(2+) at 10 micromol/L caused uniformly lower HSP72 levels. Ni(2+) exposures of 20-100 micromol/L did not induce HSP72 without heat stress, but significantly altered heat-induced HSP72 expression, with a significant increase in expression over heat alone at 40 and 100 micromol/L. Results from the current study support the hypothesis that these metal ions can, at concentrations relevant to those released from biomedical alloys, modulate HSP expression in human monocytes. The modulation of HSP expression indicates an early sign of cellular stress that may be important to the overall biological response to biomedical alloys containing and releasing these metal ions.
影响因子：
14
作者：
ANDERSON, JM;MILLER, KM
通讯作者：
MILLER, KM
金属阳离子对真核细胞代谢的体外影响。
DOI：
10.1002/jbm.820250907
发表时间：
1991
期刊：
Journal of biomedical materials research
影响因子：
--
作者：
Wataha,JC;Hanks,CT;Craig,RG
通讯作者：
Craig,RG