Exposure to metal-rich particulate matter modifies the expression of candidate microRNAs in peripheral blood leukocytes.
Exposure to metal-rich particulate matter modifies the expression of candidate microRNAs in peripheral blood leukocytes.
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DOI：
10.1289/ehp.0901300
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发表时间：
2010-06
影响因子：
10.4
通讯作者：
Baccarelli A
中科院分区：
文献类型：
--
作者：
Bollati V;Marinelli B;Apostoli P;Bonzini M;Nordio F;Hoxha M;Pegoraro V;Motta V;Tarantini L;Cantone L;Schwartz J;Bertazzi PA;Baccarelli A
基因表达模式的改变介导了颗粒物（PM）对人类健康的影响，但PM改变基因表达的机制在很大程度上尚未确定。microRNAs（miRNAs）是一类高度保守的非编码小分子RNA，在转录后水平调控广泛的基因网络的表达。我们评估了暴露于PM和PM金属成分对与电炉炼钢厂63名工人的氧化应激和炎症过程相关的候选miRNAs（miR-222，miR-21和miR-146 a）的影响。我们在工作周的第一天（基线）和工作3天后（实习后）测量了血液白细胞RNA中的miR-222、miR-21和miR-146 a表达。通过实时聚合酶链反应测量miRNA的相对表达。我们测量了血液氧化应激（8-羟基鸟嘌呤），并估计了基线和暴露后测量值之间PM 1（空气动力学直径< 1 μm）、PM10（空气动力学直径< 10 μm）、粗PM（PM10减去PM 1）和PM金属成分（铬、铅、镉、砷、镍、锰）的个体暴露。miR-222和miR-21的表达（使用2-ΔΔCT方法）在暴露后样本中显著增加（miR-222：基线= 0.68 ± 3.41，暴露后= 2.16 ± 2.25，p = 0.002; miR-21：基线= 4.10 ± 3.04，暴露后= 4.66 ± 2.63，p = 0.05）。在暴露后样本中，miR-222表达与铅暴露呈正相关（β = 0.41，p = 0.02），而miR-21表达与血液8-羟基鸟嘌呤相关（β = 0.11，p = 0.03），但与单个PM粒度级分或金属组分无关。暴露后miR-146 a的表达与基线相比没有显著差异（基线= 0.61 ± 2.42，暴露后= 1.90 ± 3.94，p = 0.19），但与铅（β =-0.51，p = 0.011）和镉（β =-0.42，p = 0.04）暴露呈负相关。miRNA表达的变化可能代表了介导对PM及其金属组分的反应的新机制。
Altered patterns of gene expression mediate the effects of particulate matter (PM) on human health, but mechanisms through which PM modifies gene expression are largely undetermined. MicroRNAs (miRNAs) are highly conserved, noncoding small RNAs that regulate the expression of broad gene networks at the posttranscriptional level. We evaluated the effects of exposure to PM and PM metal components on candidate miRNAs (miR-222, miR-21, and miR-146a) related with oxidative stress and inflammatory processes in 63 workers at an electric-furnace steel plant. We measured miR-222, miR-21, and miR-146a expression in blood leukocyte RNA on the first day of a workweek (baseline) and after 3 days of work (postexposure). Relative expression of miRNAs was measured by real-time polymerase chain reaction. We measured blood oxidative stress (8-hydroxyguanine) and estimated individual exposures to PM1 (< 1 μm in aerodynamic diameter), PM10 (< 10 μm in aerodynamic diameter), coarse PM (PM10 minus PM1), and PM metal components (chromium, lead, cadmium, arsenic, nickel, manganese) between the baseline and postexposure measurements. Expression of miR-222 and miR-21 (using the 2−ΔΔCT method) was significantly increased in postexposure samples (miR-222: baseline = 0.68 ± 3.41, postexposure = 2.16 ± 2.25, p = 0.002; miR-21: baseline = 4.10 ± 3.04, postexposure = 4.66 ± 2.63, p = 0.05). In postexposure samples, miR-222 expression was positively correlated with lead exposure (β = 0.41, p = 0.02), whereas miR-21 expression was associated with blood 8-hydroxyguanine (β = 0.11, p = 0.03) but not with individual PM size fractions or metal components. Postexposure expression of miR-146a was not significantly different from baseline (baseline = 0.61 ± 2.42, postexposure = 1.90 ± 3.94, p = 0.19) but was negatively correlated with exposure to lead (β = −0.51, p = 0.011) and cadmium (β = −0.42, p = 0.04). Changes in miRNA expression may represent a novel mechanism mediating responses to PM and its metal components.
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影响因子：
3.6
作者：
Baccarelli A;Bollati V
通讯作者：
Bollati V
影响因子：
11.2
作者：
Hurst DR;Edmonds MD;Scott GK;Benz CC;Vaidya KS;Welch DR
通讯作者：
Welch DR
影响因子：
10.4
作者：
Gurgueira SA;Lawrence J;Coull B;Murthy GG;González-Flecha B
通讯作者：
González-Flecha B
影响因子：
5
作者：
Cheng, Yunhui;Liu, Xiaojun;Zhang, Shuo;Lin, Ying;Yang, Jian;Zhang, Chunxiang
通讯作者：
Zhang, Chunxiang
影响因子：
64.8
作者：
Davis, Brandi N.;Hilyard, Aaron C.;Hata, Akiko
通讯作者：
Hata, Akiko