Rescue of Aspergillus nidulans severely debilitating null mutations in ESCRT-0, I, II and III genes by inactivation of a salt-tolerance pathway allows examination of ESCRT gene roles in pH signalling.
Rescue of Aspergillus nidulans severely debilitating null mutations in ESCRT-0, I, II and III genes by inactivation of a salt-tolerance pathway allows examination of ESCRT gene roles in pH signalling.
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DOI：
10.1242/jcs.088344
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发表时间：
2011-12-01
影响因子：
4
通讯作者：
Arst HN Jr
中科院分区：
文献类型：
--
作者：
Calcagno-Pizarelli AM;Hervás-Aguilar A;Galindo A;Abenza JF;Peñalva MA;Arst HN Jr
朋友曲霉途径将ESCRT蛋白劫持到环境pH信号复合物中。我们发现ESCRT-0、ESCRT-I、ESCRT-II和ESCRT-III的成分几乎是生长所必需的，排除了对pH信号或运输的无效突变体的评估。这种严重的衰弱效应是由两个阳离子耐受基因的功能丧失突变挽救的，其中一个是sltA，它编码一种转录因子，其失活促进高空泡化。利用条件表达sltA等位基因，我们证明了vps27 （ESCRT-0）、vps23 （ESCRT-I）、vps36 （ESCRT-II）、vps20或vps32（都是ESCRT-III）的缺失会导致许多小液泡，这种表型也被sltA下调抑制。这种情况与在酿酒酵母ESCRT零突变体中看到的正常液泡和液泡相关的E类室形成对比。利用sltA -突变的抑制表型，我们确定Vps23、Vps36、Vps20和Vps32对pH信号传导至关重要。磷脂酰肌醇3-磷酸识别蛋白Vps27 （esrt -0）在rabB无效突变体中不符合正常的pH信号，无法将Vps34激酶募集到早期内体。与缺乏Vps20或Vps32时pH信号传导的缺失相反，在缺乏ESCRT-III亚基Vps24时可检测到信号传导。我们的数据支持一个模型，其中某些ESCRT蛋白被招募到质膜来介导pH信号传导。
The Aspergillus pal pathway hijacks ESCRT proteins into ambient pH signalling complexes. We show that components of ESCRT-0, ESCRT-I, ESCRT-II and ESCRT-III are nearly essential for growth, precluding assessment of null mutants for pH signalling or trafficking. This severely debilitating effect is rescued by loss-of-function mutations in two cation tolerance genes, one of which, sltA, encodes a transcription factor whose inactivation promotes hypervacuolation. Exploiting a conditional expression sltA allele, we demonstrate that deletion of vps27 (ESCRT-0), vps23 (ESCRT-I), vps36 (ESCRT-II), or vps20 or vps32 (both ESCRT-III) leads to numerous small vacuoles, a phenotype also suppressed by SltA downregulation. This situation contrasts with normal vacuoles and vacuole-associated class E compartments seen in Saccharomyces cerevisiae ESCRT null mutants. Exploiting the suppressor phenotype of sltA− mutations, we establish that Vps23, Vps36, Vps20 and Vps32 are essential for pH signalling. Phosphatidylinositol 3-phosphate-recognising protein Vps27 (ESCRT-0) is not, consistent with normal pH signalling in rabB null mutants unable to recruit Vps34 kinase to early endosomes. In contrast to the lack of pH signalling in the absence of Vps20 or Vps32, detectable signalling occurs in the absence of ESCRT-III subunit Vps24. Our data support a model in which certain ESCRT proteins are recruited to the plasma membrane to mediate pH signalling.
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DOI：
10.1038/nrm2937
发表时间：
2010-08
期刊：
Nature reviews. Molecular cell biology
影响因子：
--
作者：
通讯作者：
--
影响因子：
3
作者：
Findon, Helen;Calcagno-Pizarelli, Ana-Maria;Martinez, Jose L.;Spielvogel, Anja;Markina-Inarrairaegui, Ane;Indrakumar, Tanya;Ramos, Jose;Penalva, Miguel A.;Espeso, Eduardo A.;Arst, Herbert N., Jr.
通讯作者：
Arst, Herbert N., Jr.
影响因子：
3
作者：
Hervas-Aguilar, America;Rodriguez-Galan, Olga;Galindo, Antonio;Abenza, Juan F.;Arst, Herbert N., Jr.;Penalva, Miguel A.
通讯作者：
Penalva, Miguel A.
影响因子：
4.5
作者：
Abenza, Juan F.;Pantazopoulou, Areti;Penalva, Miguel A.
通讯作者：
Penalva, Miguel A.
影响因子：
--
作者：
Apostolaki, Angeliki;Erpapazoglou, Zoi;Sophianopoulou, Vicky
通讯作者：
Sophianopoulou, Vicky