Characterization of Aspergillus nidulans DidB Did2, a non-essential component of the multivesicular body pathway.
Characterization of Aspergillus nidulans DidB Did2, a non-essential component of the multivesicular body pathway.
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DOI：
10.1016/j.fgb.2010.03.010
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发表时间：
2010-07
影响因子：
3
通讯作者：
Penalva, Miguel A.
中科院分区：
文献类型：
--
作者：
Hervas-Aguilar, America;Rodriguez-Galan, Olga;Galindo, Antonio;Abenza, Juan F.;Arst, Herbert N., Jr.;Penalva, Miguel A.
ESCRT-III异质聚合物介导膜蛋白货物分选进入多泡内体，随后进行空泡降解。我们利用其关键结构成分Vps32和“相关”成分DidBDid2研究了很大程度上未被表征的空心曲霉ESCRT-III的定位。据报道，Vps32-GFP定位于运动的早期核内体，但由于无法与核内体分离，Vps32-GFP在通常与液泡相关的聚集体中占主导地位。DidBDid2调节Vps4 （atp酶分解ESCRT-III）不是必需的。与这种辅助作用一致，didBΔ不能阻断谷氨酸转运体AgtA的MVB分选，但在促进其液泡靶向的条件下，增加其稳态水平并将一部分渗透酶错定位到质膜上。didBΔ加剧了Vps32-GFP过表达导致的显性负生长缺陷。在与RabARab5共定位的早期核内体中可以检测到一定比例的DidB-GFP，并在nudA1顶端积累，这表明ESCRT-III从内吞噬途径的早期阶段就组装在核内体上。
ESCRT-III heteropolymers mediate membrane protein cargo sorting into multivesicular endosomes for subsequent vacuolar degradation. We studied the localization of largely uncharacterized Aspergillus nidulans ESCRT-III using its key structural component Vps32 and the ‘associated’ component DidBDid2. Vps32-GFP localizes to motile early endosomes as reported, but predominates in aggregates often associated with vacuoles due to inability to dissociate from endosomes. DidBDid2 regulating Vps4 (the ATPase disassembling ESCRT-III) is not essential. Consistent with this accessory role, didBΔ is unable to block the MVB sorting of the glutamate transporter AgtA, but increases its steady-state level and mislocalizes a fraction of the permease to the plasma membrane under conditions promoting its vacuolar targeting. didBΔ exacerbates the dominant-negative growth defect resulting from Vps32-GFP over-expression. A proportion of DidB-GFP is detectable in early endosomes colocalizing with RabARab5 and accumulating in nudA1 tips, suggesting that ESCRT-III assembles on endosomes from the early steps of the endocytic pathway.
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DOI：
10.1083/jcb.200508166
发表时间：
2006-02-27
期刊：
The Journal of cell biology
影响因子：
--
作者：
Azmi I;Davies B;Dimaano C;Payne J;Eckert D;Babst M;Katzmann DJ
通讯作者：
Katzmann DJ
影响因子：
64.5
作者：
Katzmann, DJ;Babst, M;Emr, SD
通讯作者：
Emr, SD
影响因子：
56.9
作者：
Carlton, Jez G.;Martin-Serrano, Juan
通讯作者：
Martin-Serrano, Juan
影响因子：
3.3
作者：
Amerik, AY;Nowak, J;Hochstrasser, M
通讯作者：
Hochstrasser, M
影响因子：
4.5
作者：
Abenza, Juan F.;Pantazopoulou, Areti;Penalva, Miguel A.
通讯作者：
Penalva, Miguel A.